Potassium in PDB 2a1m: Crystal Structure of Ferrous Dioxygen Complex of Wild-Type Cytochrome P450CAM

All present enzymatic activity of Crystal Structure of Ferrous Dioxygen Complex of Wild-Type Cytochrome P450CAM:
1.14.15.1;

The structure of Crystal Structure of Ferrous Dioxygen Complex of Wild-Type Cytochrome P450CAM, PDB code: 2a1m was solved by S.Nagano, T.L.Poulos, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.82 / 2.10
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	67.060, 62.290, 95.640, 90.00, 90.42, 90.00
R / Rfree (%)	19.1 / 24.7

The structure of Crystal Structure of Ferrous Dioxygen Complex of Wild-Type Cytochrome P450CAM also contains other interesting chemical elements:

Iron

(Fe)

2 atoms

The binding sites of Potassium atom in the Crystal Structure of Ferrous Dioxygen Complex of Wild-Type Cytochrome P450CAM (pdb code 2a1m). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 3 binding sites of Potassium where determined in the Crystal Structure of Ferrous Dioxygen Complex of Wild-Type Cytochrome P450CAM, PDB code: 2a1m:
Jump to Potassium binding site number: 1; 2; 3;

Potassium binding site 1 out of 3 in the Crystal Structure of Ferrous Dioxygen Complex of Wild-Type Cytochrome P450CAM


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Ferrous Dioxygen Complex of Wild-Type Cytochrome P450CAM within 5.0Å range: probe atom residue distance (Å) B Occ A:K1515 b:17.0 occ:1.00 O A:GLU84 2.8 11.2 1.0 O A:TYR96 2.9 13.4 1.0 O A:GLU94 3.0 15.7 1.0 O A:GLY93 3.0 15.1 1.0 C A:GLU94 3.5 14.5 1.0 CA A:GLU94 3.7 11.5 1.0 C A:TYR96 3.8 15.0 1.0 C A:GLU84 4.0 15.3 1.0 C A:GLY93 4.0 11.8 1.0 N A:TYR96 4.2 15.2 1.0 N A:GLU94 4.3 11.2 1.0 CA A:TYR96 4.4 15.5 1.0 N A:ALA95 4.4 13.6 1.0 C A:ALA95 4.5 16.1 1.0 CG A:GLU84 4.6 26.7 1.0 CA A:CYS85 4.6 12.6 1.0 CB A:TYR96 4.6 14.1 1.0 N A:CYS85 4.7 11.7 1.0 N A:ASP97 4.8 16.1 1.0 CB A:GLU94 4.9 12.0 1.0 CA A:ASP97 4.9 15.0 1.0 CA A:ALA95 5.0 15.4 1.0 CA A:GLU84 5.0 15.0 1.0

Potassium binding site 2 out of 3 in the Crystal Structure of Ferrous Dioxygen Complex of Wild-Type Cytochrome P450CAM


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Ferrous Dioxygen Complex of Wild-Type Cytochrome P450CAM within 5.0Å range: probe atom residue distance (Å) B Occ A:K1715 b:24.9 occ:1.00 O A:VAL18 2.9 18.2 1.0 O A:HOH1718 3.0 24.1 1.0 O A:PRO15 3.0 21.9 1.0 O A:HOH1882 3.1 16.6 1.0 OE2 A:GLU20 3.1 23.0 1.0 O A:PRO16 3.2 14.9 1.0 C A:PRO16 3.5 18.3 1.0 O A:HOH1964 3.7 23.4 1.0 CA A:PRO16 3.7 20.9 1.0 C A:VAL18 3.7 16.3 1.0 CD2 A:LEU14 3.8 20.5 1.0 CD A:GLU20 3.9 22.2 1.0 C A:PRO15 4.0 22.7 1.0 N A:VAL18 4.0 13.6 1.0 CG A:LEU14 4.0 14.8 1.0 OE1 A:GLU20 4.2 19.5 1.0 N A:PRO16 4.3 21.6 1.0 CA A:VAL18 4.4 14.1 1.0 N A:HIS17 4.4 17.1 1.0 O A:HOH1720 4.4 38.9 1.0 C A:HIS17 4.6 15.6 1.0 O A:LEU14 4.6 22.2 1.0 N A:PRO19 4.7 17.6 1.0 CB A:VAL18 4.9 12.6 1.0 CA A:PRO19 4.9 19.3 1.0 N A:GLU20 4.9 19.7 1.0 CA A:HIS17 4.9 17.2 1.0

Potassium binding site 3 out of 3 in the Crystal Structure of Ferrous Dioxygen Complex of Wild-Type Cytochrome P450CAM


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Crystal Structure of Ferrous Dioxygen Complex of Wild-Type Cytochrome P450CAM within 5.0Å range: probe atom residue distance (Å) B Occ B:K2515 b:24.8 occ:1.00 O B:TYR96 2.9 19.7 1.0 O B:GLU84 3.0 25.7 1.0 O B:HOH2582 3.1 23.9 1.0 O B:GLU94 3.1 20.5 1.0 O B:GLY93 3.2 23.4 1.0 C B:GLU94 3.7 19.8 1.0 CA B:GLU94 3.9 21.6 1.0 C B:TYR96 3.9 21.1 1.0 C B:GLU84 4.0 24.1 1.0 CG B:GLU84 4.2 39.9 1.0 C B:GLY93 4.3 22.3 1.0 N B:TYR96 4.4 21.1 1.0 CA B:TYR96 4.6 20.2 1.0 N B:GLU94 4.6 21.6 1.0 N B:ALA95 4.6 20.2 1.0 C B:ALA95 4.7 20.0 1.0 CA B:CYS85 4.8 19.9 1.0 N B:CYS85 4.8 21.1 1.0 CB B:TYR96 4.8 20.1 1.0 N B:ASP97 4.9 20.9 1.0 CA B:GLU84 4.9 24.7 1.0

S.Nagano, T.L.Poulos. Crystallographic Study on the Dioxygen Complex of Wild-Type and Mutant Cytochrome P450CAM. Implications For the Dioxygen Activation Mechanism J.Biol.Chem. V. 280 31659 2005.
ISSN: ISSN 0021-9258
PubMed: 15994329
DOI: 10.1074/JBC.M505261200