Potassium in PDB 1zqp: Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Kcl (75 Millimolar) and Nacl (75 Millimolar)

The structure of Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Kcl (75 Millimolar) and Nacl (75 Millimolar), PDB code: 1zqp was solved by H.Pelletier, M.R.Sawaya, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.80
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	178.112, 57.710, 48.173, 90.00, 90.00, 90.00
R / Rfree (%)	16.6 / n/a

The binding sites of Potassium atom in the Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Kcl (75 Millimolar) and Nacl (75 Millimolar) (pdb code 1zqp). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Kcl (75 Millimolar) and Nacl (75 Millimolar), PDB code: 1zqp:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Kcl (75 Millimolar) and Nacl (75 Millimolar)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Kcl (75 Millimolar) and Nacl (75 Millimolar) within 5.0Å range: probe atom residue distance (Å) B Occ A:K341 b:31.0 occ:1.00 O A:THR101 2.1 15.3 1.0 OP1 P:DT6 2.4 11.9 1.0 O A:ILE106 2.6 13.9 1.0 O A:VAL103 2.7 9.1 1.0 O P:HOH601 2.8 0.0 1.0 O P:HOH665 3.1 71.4 1.0 C A:THR101 3.4 13.9 1.0 P P:DT6 3.4 1.6 1.0 OP2 P:DT6 3.5 1.0 1.0 C A:VAL103 3.6 9.7 1.0 C A:ILE106 3.7 12.9 1.0 N A:GLY105 3.9 11.9 1.0 CA A:SER104 3.9 8.0 1.0 N A:ILE106 4.0 8.1 1.0 N A:SER104 4.1 8.4 1.0 C A:ARG102 4.2 27.2 1.0 N A:VAL103 4.2 24.6 1.0 CA A:THR101 4.2 6.5 1.0 N A:ARG102 4.3 19.4 1.0 CA A:ILE106 4.3 9.3 1.0 C A:SER104 4.4 8.9 1.0 CA A:ARG102 4.4 26.2 1.0 O3' P:DA5 4.5 5.5 1.0 O A:ARG102 4.6 30.7 1.0 O5' P:DT6 4.6 5.8 1.0 CA A:VAL103 4.6 17.3 1.0 CB A:ILE106 4.6 2.4 1.0 N A:GLY107 4.7 14.8 1.0 CG2 A:THR101 4.8 8.5 1.0 C A:GLY105 5.0 8.1 1.0

Potassium binding site 2 out of 2 in the Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Kcl (75 Millimolar) and Nacl (75 Millimolar)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Kcl (75 Millimolar) and Nacl (75 Millimolar) within 5.0Å range: probe atom residue distance (Å) B Occ A:K342 b:60.8 occ:1.00 O A:LEU62 2.2 51.4 1.0 O A:LYS60 2.8 29.5 1.0 O A:VAL65 3.2 21.1 1.0 C A:LEU62 3.4 41.5 1.0 C A:LYS60 4.0 23.4 1.0 C A:VAL65 4.0 18.2 1.0 N A:VAL65 4.1 19.0 1.0 N A:LEU62 4.2 28.6 1.0 C A:LYS61 4.2 35.9 1.0 N A:PRO63 4.3 35.7 1.0 CA A:PRO63 4.3 33.5 1.0 CA A:VAL65 4.4 17.5 1.0 CA A:LEU62 4.4 34.6 1.0 N A:GLY64 4.4 43.1 1.0 O A:LYS61 4.6 42.3 1.0 CA A:LYS61 4.7 37.9 1.0 CB A:VAL65 4.7 26.1 1.0 C A:PRO63 4.7 40.2 1.0 CG A:LYS60 4.8 34.1 1.0 N A:LYS61 4.8 29.0 1.0 C A:GLY64 5.0 31.9 1.0 CA A:LYS60 5.0 21.4 1.0

H.Pelletier, M.R.Sawaya. Characterization of the Metal Ion Binding Helix-Hairpin-Helix Motifs in Human Dna Polymerase Beta By X-Ray Structural Analysis. Biochemistry V. 35 12778 1996.
ISSN: ISSN 0006-2960
PubMed: 8841120
DOI: 10.1021/BI960790I