Potassium in PDB 1zqi: Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Kcl (150 Millimolar)

The structure of Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Kcl (150 Millimolar), PDB code: 1zqi was solved by H.Pelletier, M.R.Sawaya, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.70
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	178.497, 57.809, 48.358, 90.00, 90.00, 90.00
R / Rfree (%)	17.9 / n/a

The binding sites of Potassium atom in the Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Kcl (150 Millimolar) (pdb code 1zqi). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Kcl (150 Millimolar), PDB code: 1zqi:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Kcl (150 Millimolar)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Kcl (150 Millimolar) within 5.0Å range: probe atom residue distance (Å) B Occ A:K341 b:16.0 occ:1.00 O A:THR101 2.3 28.3 1.0 OP1 P:DT6 2.3 17.1 1.0 O A:ILE106 2.5 16.2 1.0 O A:VAL103 2.6 13.8 1.0 O P:HOH601 2.6 96.2 1.0 O P:HOH665 2.9 36.4 1.0 P P:DT6 3.4 6.7 1.0 C A:THR101 3.5 21.6 1.0 C A:ILE106 3.7 10.4 1.0 C A:VAL103 3.7 11.7 1.0 OP2 P:DT6 3.7 2.9 1.0 N A:GLY105 3.9 13.3 1.0 N A:ILE106 3.9 4.3 1.0 CA A:SER104 4.1 14.0 1.0 C A:ARG102 4.3 27.3 1.0 N A:VAL103 4.3 19.1 1.0 CA A:THR101 4.3 16.2 1.0 CA A:ILE106 4.3 5.6 1.0 N A:ARG102 4.4 20.3 1.0 O3' P:DA5 4.4 10.1 1.0 N A:SER104 4.4 11.7 1.0 CA A:ARG102 4.5 23.2 1.0 C A:SER104 4.5 15.6 1.0 O5' P:DT6 4.6 10.5 1.0 N A:GLY107 4.6 11.7 1.0 O A:ARG102 4.7 35.8 1.0 CB A:ILE106 4.7 2.0 1.0 CA A:VAL103 4.7 12.8 1.0 C A:GLY105 4.9 6.6 1.0 CA A:GLY105 4.9 11.6 1.0 CA A:GLY107 4.9 12.2 1.0 CG2 A:THR101 4.9 15.7 1.0

Potassium binding site 2 out of 2 in the Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Kcl (150 Millimolar)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Kcl (150 Millimolar) within 5.0Å range: probe atom residue distance (Å) B Occ A:K342 b:65.2 occ:1.00 O A:LEU62 2.3 51.1 1.0 O A:LYS60 3.0 38.2 1.0 O A:VAL65 3.2 22.9 1.0 C A:LEU62 3.5 40.4 1.0 C A:VAL65 4.1 19.1 1.0 C A:LYS60 4.1 32.6 1.0 N A:LEU62 4.2 31.0 1.0 C A:LYS61 4.2 33.2 1.0 N A:VAL65 4.2 24.3 1.0 N A:PRO63 4.4 35.0 1.0 CA A:PRO63 4.4 33.5 1.0 CA A:LEU62 4.5 33.0 1.0 O A:LYS61 4.5 36.6 1.0 N A:GLY64 4.5 40.0 1.0 CA A:VAL65 4.5 19.4 1.0 CA A:LYS61 4.7 32.5 1.0 CB A:VAL65 4.8 25.3 1.0 N A:LYS61 4.9 30.3 1.0 CG A:LYS60 4.9 43.8 1.0 C A:PRO63 4.9 40.4 1.0

H.Pelletier, M.R.Sawaya. Characterization of the Metal Ion Binding Helix-Hairpin-Helix Motifs in Human Dna Polymerase Beta By X-Ray Structural Analysis. Biochemistry V. 35 12778 1996.
ISSN: ISSN 0006-2960
PubMed: 8841120
DOI: 10.1021/BI960790I