Potassium in the structure of Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed With Seven Base Pairs Of Dna; Soaked in the Presence of Kcl (150 Millimolar) At pH 7.5 (pdb 1zqa)

The binding sites of Potassium atom in the structure of Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed With Seven Base Pairs Of Dna; Soaked in the Presence of Kcl (150 Millimolar) At pH 7.5 (pdb code 1zqa). This binding sites where shown with 5.0 Angstroms radius around Potassium atom. The 1zqa structure was solved by H.PELLETIER, M.R.SAWAYA, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 20.0-3.2 Space group P21212 a (A) 178.376 b (A) 57.899 c (A) 48.433 alpha (°) 90.00 beta (°) 90.00 gamma (°) 90.00 Rfactor (%) 14.6 Rfree (%) n/a Potassium Binding Sites: Potassium binding site 1 out of 2 in 1zqa Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Potassium in the PDB 1zqa. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Potassium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: P: Da5, P: Dt6, A: Thr101, A: Arg102, A: Val103, A: Ser104, A: Gly105, A: Ile106, A: Gly107, P: Hoh665, A: Hoh601, conact list: Atom Atom Distance (A) K O3' P:Da5 4.43 K OP1 P:Dt6 2.13 K P P:Dt6 3.42 K OP2 P:Dt6 3.80 K O5' P:Dt6 4.59 K O A:Thr101 2.40 K C A:Thr101 3.53 K CA A:Thr101 4.32 K O A:Arg102 4.53 K N A:Arg102 4.47 K C A:Arg102 4.23 K CA A:Arg102 4.55 K O A:Val103 2.63 K N A:Val103 4.25 K C A:Val103 3.55 K CA A:Val103 4.59 K N A:Ser104 4.13 K C A:Ser104 4.37 K CA A:Ser104 3.97 K N A:Gly105 3.88 K C A:Gly105 4.89 K CA A:Gly105 4.94 K O A:Ile106 2.69 K N A:Ile106 3.87 K CB A:Ile106 4.70 K C A:Ile106 3.75 K CA A:Ile106 4.32 K N A:Gly107 4.80 K O P:Hoh665 2.94 K O A:Hoh601 2.83 interactive model: Potassium binding site 2 out of 2 in 1zqa Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Potassium in the PDB 1zqa. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Potassium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Lys60, A: Lys61, A: Leu62, A: Pro63, A: Gly64, A: Val65, conact list: Atom Atom Distance (A) K O A:Lys60 2.92 K C A:Lys60 4.04 K O A:Lys61 4.17 K N A:Lys61 4.68 K C A:Lys61 3.99 K CA A:Lys61 4.44 K O A:Leu62 2.32 K N A:Leu62 4.10 K C A:Leu62 3.41 K CA A:Leu62 4.39 K N A:Pro63 4.20 K C A:Pro63 4.84 K CA A:Pro63 4.26 K N A:Gly64 4.56 K O A:Val65 3.32 K N A:Val65 4.44 K C A:Val65 4.25 K CA A:Val65 4.73 interactive model: