Potassium in PDB 1xl4: Intermediate Gating Structure 1 of the Inwardly Rectifying K+ Channel KIRBAC3.1

The structure of Intermediate Gating Structure 1 of the Inwardly Rectifying K+ Channel KIRBAC3.1, PDB code: 1xl4 was solved by J.M.Gulbis, A.Kuo, B.Smith, D.A.Doyle, A.Edwards, C.Arrowsmith, M.Sundstrom, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	12.00 / 2.60
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	105.627, 109.385, 88.786, 90.00, 90.00, 90.00
R / Rfree (%)	22.6 / 25.3

The structure of Intermediate Gating Structure 1 of the Inwardly Rectifying K+ Channel KIRBAC3.1 also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Potassium atom in the Intermediate Gating Structure 1 of the Inwardly Rectifying K+ Channel KIRBAC3.1 (pdb code 1xl4). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 5 binding sites of Potassium where determined in the Intermediate Gating Structure 1 of the Inwardly Rectifying K+ Channel KIRBAC3.1, PDB code: 1xl4:
Jump to Potassium binding site number: 1; 2; 3; 4; 5;

Potassium binding site 1 out of 5 in the Intermediate Gating Structure 1 of the Inwardly Rectifying K+ Channel KIRBAC3.1


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Intermediate Gating Structure 1 of the Inwardly Rectifying K+ Channel KIRBAC3.1 within 5.0Å range:

Potassium binding site 2 out of 5 in the Intermediate Gating Structure 1 of the Inwardly Rectifying K+ Channel KIRBAC3.1


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Intermediate Gating Structure 1 of the Inwardly Rectifying K+ Channel KIRBAC3.1 within 5.0Å range: probe atom residue distance (Å) B Occ A:K1001 b:45.2 occ:0.50 O A:TYR99 4.5 55.2 1.0 O B:TYR99 4.6 57.0 1.0 CA A:GLY100 4.7 63.1 1.0 K A:K1002 4.8 44.9 0.5 CA B:GLY100 4.9 64.2 1.0

Potassium binding site 3 out of 5 in the Intermediate Gating Structure 1 of the Inwardly Rectifying K+ Channel KIRBAC3.1


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Intermediate Gating Structure 1 of the Inwardly Rectifying K+ Channel KIRBAC3.1 within 5.0Å range: probe atom residue distance (Å) B Occ A:K1002 b:44.9 occ:0.50 O A:HOH2002 0.9 53.4 0.5 O A:TYR99 2.4 55.2 1.0 O B:TYR99 2.5 57.0 1.0 O A:HOH2003 2.8 55.9 0.5 O A:GLY98 2.9 45.1 1.0 O B:GLY98 3.0 51.1 1.0 C A:TYR99 3.4 55.7 1.0 C B:TYR99 3.5 57.9 1.0 K A:K1003 3.9 14.5 0.5 C A:GLY98 4.1 48.1 1.0 C B:GLY98 4.2 52.1 1.0 CA A:TYR99 4.2 52.8 1.0 CA B:TYR99 4.2 55.4 1.0 N B:GLY100 4.3 59.9 1.0 N A:GLY100 4.3 58.4 1.0 CA B:GLY100 4.4 64.2 1.0 CA A:GLY100 4.4 63.1 1.0 N A:TYR99 4.7 49.2 1.0 N B:TYR99 4.7 53.5 1.0 K A:K1001 4.8 45.2 0.5

Potassium binding site 4 out of 5 in the Intermediate Gating Structure 1 of the Inwardly Rectifying K+ Channel KIRBAC3.1


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Intermediate Gating Structure 1 of the Inwardly Rectifying K+ Channel KIRBAC3.1 within 5.0Å range: probe atom residue distance (Å) B Occ A:K1003 b:14.5 occ:0.50 O A:HOH2003 1.0 55.9 0.5 O A:ILE97 2.7 39.6 1.0 O B:ILE97 2.7 46.6 1.0 O A:HOH2002 3.0 53.4 0.5 O A:GLY98 3.0 45.1 1.0 O B:GLY98 3.1 51.1 1.0 C B:ILE97 3.7 46.6 1.0 C A:GLY98 3.7 48.1 1.0 C B:GLY98 3.7 52.1 1.0 C A:ILE97 3.7 42.2 1.0 K A:K1002 3.9 44.9 0.5 CA A:GLY98 4.0 46.7 1.0 CA B:GLY98 4.0 50.0 1.0 O B:HOH2002 4.0 34.7 0.5 N B:GLY98 4.3 47.4 1.0 N A:GLY98 4.3 43.0 1.0 O A:THR96 4.4 39.3 1.0 O B:THR96 4.5 40.5 1.0 N B:TYR99 4.7 53.5 1.0 N A:TYR99 4.8 49.2 1.0 CA B:ILE97 4.8 44.1 1.0 CA A:ILE97 4.9 40.9 1.0

Potassium binding site 5 out of 5 in the Intermediate Gating Structure 1 of the Inwardly Rectifying K+ Channel KIRBAC3.1


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 5 of Intermediate Gating Structure 1 of the Inwardly Rectifying K+ Channel KIRBAC3.1 within 5.0Å range: probe atom residue distance (Å) B Occ A:K1004 b:27.7 occ:0.50 MG A:MG2001 0.9 44.1 0.5 O B:HOH2002 1.2 34.7 0.5 OG1 A:THR96 2.5 38.4 1.0 O B:THR96 2.6 40.5 1.0 O A:THR96 2.6 39.3 1.0 OG1 B:THR96 2.7 36.5 1.0 CB A:THR96 3.5 38.7 1.0 C B:THR96 3.6 40.3 1.0 CB B:THR96 3.6 36.8 1.0 C A:THR96 3.6 39.0 1.0 CA A:THR96 4.2 39.0 1.0 CA B:THR96 4.3 38.6 1.0 N B:ILE97 4.5 41.8 1.0 O B:ALA95 4.6 36.5 1.0 N A:ILE97 4.6 39.3 1.0 O A:ALA95 4.6 37.9 1.0 CG2 A:THR96 4.7 38.0 1.0 CA B:ILE97 4.7 44.1 1.0 CA A:ILE97 4.8 40.9 1.0 CG2 B:THR96 4.9 34.1 1.0

J.M.Gulbis, A.Kuo, B.Smith, D.A.Doyle, A.Edwards, C.Arrowsmith, M.Sundstrom. Two Intermediate Gating State Crystal Structures of the KIRBAC3.1 K+ Channel To Be Published.