Potassium in PDB 1wcf: 1.54 A Crystal Structure of RV3628, Mycobacterium Tuberculosis Inorganic Pyrophosphatase (Ppase) at PH7.0

All present enzymatic activity of 1.54 A Crystal Structure of RV3628, Mycobacterium Tuberculosis Inorganic Pyrophosphatase (Ppase) at PH7.0:
3.6.1.1;

The structure of 1.54 A Crystal Structure of RV3628, Mycobacterium Tuberculosis Inorganic Pyrophosphatase (Ppase) at PH7.0, PDB code: 1wcf was solved by S.Benini, K.S.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	84.52 / 1.54
Space group	P 63 2 2
Cell size a, b, c (Å), α, β, γ (°)	96.902, 96.902, 103.312, 90.00, 90.00, 120.00
R / Rfree (%)	15.6 / 17

The binding sites of Potassium atom in the 1.54 A Crystal Structure of RV3628, Mycobacterium Tuberculosis Inorganic Pyrophosphatase (Ppase) at PH7.0 (pdb code 1wcf). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the 1.54 A Crystal Structure of RV3628, Mycobacterium Tuberculosis Inorganic Pyrophosphatase (Ppase) at PH7.0, PDB code: 1wcf:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the 1.54 A Crystal Structure of RV3628, Mycobacterium Tuberculosis Inorganic Pyrophosphatase (Ppase) at PH7.0


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of 1.54 A Crystal Structure of RV3628, Mycobacterium Tuberculosis Inorganic Pyrophosphatase (Ppase) at PH7.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:K1162 b:22.8 occ:1.00 O A:GLU130 2.6 22.7 1.0 O A:LYS133 2.7 24.2 1.0 O A:HOH2236 2.7 30.9 1.0 O A:LYS127 2.8 15.9 1.0 O A:ASP128 3.0 20.0 1.0 O A:HOH2229 3.3 48.5 1.0 C A:ASP128 3.5 17.6 1.0 C A:GLU130 3.6 21.8 1.0 C A:LYS133 3.8 23.4 1.0 C A:LYS127 3.9 15.5 1.0 CA A:ASP128 3.9 16.7 1.0 N A:GLU130 3.9 20.3 1.0 N A:LYS133 4.1 24.3 1.0 CA A:GLU130 4.3 20.7 1.0 N A:LEU129 4.3 17.2 1.0 N A:ASP128 4.4 15.5 1.0 O A:HOH2227 4.5 29.7 1.0 CA A:LYS133 4.5 24.0 1.0 N A:PRO131 4.5 23.6 1.0 O A:HOH2228 4.5 45.1 1.0 N A:GLY132 4.6 24.2 1.0 O A:HOH2233 4.6 52.5 1.0 CA A:PRO131 4.6 24.3 1.0 C A:LEU129 4.7 20.2 1.0 CB A:LYS133 4.8 23.6 1.0 C A:PRO131 4.8 24.4 1.0 O A:HOH2114 4.8 48.9 1.0 CB A:GLU130 4.8 20.5 1.0 N A:PHE134 4.9 22.7 1.0 CA A:LEU129 4.9 18.4 1.0

Potassium binding site 2 out of 2 in the 1.54 A Crystal Structure of RV3628, Mycobacterium Tuberculosis Inorganic Pyrophosphatase (Ppase) at PH7.0


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of 1.54 A Crystal Structure of RV3628, Mycobacterium Tuberculosis Inorganic Pyrophosphatase (Ppase) at PH7.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:K1163 b:34.7 occ:1.00 O A:HOH2180 2.7 16.2 1.0 O A:HOH2147 2.7 28.2 1.0 O A:HOH2140 2.8 19.0 1.0 OD1 A:ASP89 2.9 33.3 1.0 O A:HOH2144 3.1 28.0 1.0 OD1 A:ASP57 3.3 21.4 1.0 O A:HOH2153 3.4 35.5 1.0 O A:HOH2152 3.4 35.9 1.0 CG A:ASP57 3.6 20.7 1.0 O A:PRO55 3.6 15.8 1.0 OD2 A:ASP57 3.6 26.3 1.0 O A:HOH2056 4.1 21.8 1.0 OD2 A:ASP52 4.1 23.1 1.0 CG A:ASP89 4.1 17.9 1.0 O A:HOH2178 4.2 36.5 1.0 N A:ASP57 4.4 13.5 1.0 CB A:ASP57 4.5 15.7 1.0 CB A:ASP54 4.6 20.3 1.0 OD1 A:ASP52 4.7 21.1 1.0 C A:PRO55 4.8 15.1 1.0 CA A:ASP89 4.8 21.9 1.0 CG A:ASP52 4.8 21.4 1.0 CB A:ASP89 5.0 24.2 1.0

S.Benini, K.S.Wilson. Structure of the Mycobacterium Tuberculosis Soluble Inorganic Pyrophosphatase RV3628 at pH 7.0. Acta Crystallogr.,Sect.F V. 67 866 2011.
ISSN: ISSN 1744-3091
PubMed: 21821883
DOI: 10.1107/S1744309111023323