Potassium in PDB 1v1m: Crosstalk Between Cofactor Binding and the Phosphorylation Loop Conformation in the Bckd Machine

All present enzymatic activity of Crosstalk Between Cofactor Binding and the Phosphorylation Loop Conformation in the Bckd Machine:
1.2.4.4;

The structure of Crosstalk Between Cofactor Binding and the Phosphorylation Loop Conformation in the Bckd Machine, PDB code: 1v1m was solved by J.Li, R.M.Wynn, M.Machius, J.L.Chuang, S.Karthikeyan, D.R.Tomchick, D.T.Chuang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.87 / 2.00
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	145.287, 145.287, 69.165, 90.00, 90.00, 120.00
R / Rfree (%)	12.9 / 17

The structure of Crosstalk Between Cofactor Binding and the Phosphorylation Loop Conformation in the Bckd Machine also contains other interesting chemical elements:

Manganese	(Mn)	1 atom
Chlorine	(Cl)	3 atoms

The binding sites of Potassium atom in the Crosstalk Between Cofactor Binding and the Phosphorylation Loop Conformation in the Bckd Machine (pdb code 1v1m). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crosstalk Between Cofactor Binding and the Phosphorylation Loop Conformation in the Bckd Machine, PDB code: 1v1m:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crosstalk Between Cofactor Binding and the Phosphorylation Loop Conformation in the Bckd Machine


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crosstalk Between Cofactor Binding and the Phosphorylation Loop Conformation in the Bckd Machine within 5.0Å range: probe atom residue distance (Å) B Occ A:K1404 b:19.6 occ:1.00 O A:PRO163 2.7 6.0 1.0 O A:HOH2103 2.7 10.1 1.0 O A:SER161 2.8 9.0 1.0 OE1 A:GLN167 2.9 8.0 1.0 OG A:SER161 2.9 7.2 1.0 OE1 A:GLN112 3.0 13.4 1.0 OG1 A:THR166 3.0 8.8 1.0 C A:SER161 3.7 8.1 1.0 C A:PRO163 3.7 7.8 1.0 CD A:GLN112 3.7 11.4 1.0 CD A:GLN167 3.9 6.1 1.0 CB A:SER161 4.1 8.7 1.0 N A:SER161 4.1 7.6 1.0 O B:HOH2102 4.1 9.9 1.0 CA A:SER161 4.2 8.2 1.0 N A:GLN167 4.3 7.7 1.0 N A:THR166 4.3 7.4 1.0 CB A:THR166 4.4 8.9 1.0 N A:PRO163 4.4 8.6 1.0 CG A:GLN112 4.4 14.0 1.0 N A:ALA165 4.5 6.8 1.0 C A:SER162 4.5 8.1 1.0 CB A:GLN167 4.5 7.2 1.0 NE2 A:GLN112 4.5 14.3 1.0 CA A:LEU164 4.5 8.1 1.0 N A:LEU164 4.6 8.0 1.0 O A:GLY111 4.6 13.4 1.0 CA A:PRO163 4.6 7.9 1.0 O A:HOH2165 4.6 13.6 1.0 N A:SER162 4.6 6.7 1.0 C A:LEU164 4.7 7.1 1.0 CA A:THR166 4.7 8.4 1.0 CG A:GLN167 4.7 7.0 1.0 NE2 A:GLN167 4.8 5.8 1.0 CB A:GLN112 4.8 14.7 1.0 CD A:PRO163 4.8 8.3 1.0 O A:HOH2104 4.8 11.9 1.0 OE2 B:GLU113 4.8 6.1 1.0 O A:SER162 4.8 7.9 1.0 C A:THR166 4.9 7.9 1.0

Potassium binding site 2 out of 2 in the Crosstalk Between Cofactor Binding and the Phosphorylation Loop Conformation in the Bckd Machine


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crosstalk Between Cofactor Binding and the Phosphorylation Loop Conformation in the Bckd Machine within 5.0Å range: probe atom residue distance (Å) B Occ B:K2347 b:24.3 occ:1.00 O B:ASN183 2.7 7.3 1.0 O B:CYS178 2.7 8.7 1.0 O B:GLY128 2.8 9.3 1.0 O B:ASP181 2.9 9.3 1.0 O B:LEU130 3.0 7.5 1.0 O B:HOH2118 3.0 14.3 1.0 OG1 B:THR131 3.5 8.5 1.0 C B:ASN183 3.8 8.4 1.0 C B:LEU130 3.8 7.9 1.0 C B:ASP181 3.9 8.3 1.0 C B:CYS178 3.9 10.0 1.0 C B:GLY128 3.9 9.2 1.0 O B:ILE179 4.0 8.9 1.0 N B:LEU130 4.1 8.1 1.0 SG B:CYS185 4.1 10.1 1.0 O B:HOH2152 4.2 9.9 0.5 C B:SER129 4.2 9.4 1.0 N B:ASN183 4.2 9.4 1.0 N B:CYS185 4.2 7.3 1.0 N B:ASP181 4.3 8.9 1.0 C B:ILE179 4.3 9.6 1.0 CA B:SER129 4.4 8.8 1.0 CB B:ASP181 4.4 9.6 1.0 CB B:CYS185 4.4 9.3 1.0 CA B:ASP181 4.4 8.8 1.0 CA B:PRO184 4.5 7.0 1.0 N B:THR131 4.5 7.7 1.0 N B:PRO184 4.5 7.3 1.0 CA B:LEU130 4.6 8.5 1.0 CA B:ILE179 4.6 9.4 1.0 N B:SER129 4.6 8.6 1.0 CA B:ASN183 4.7 7.8 1.0 C B:PRO184 4.7 6.7 1.0 N B:ILE179 4.7 9.0 1.0 CB B:THR131 4.7 8.4 1.0 O B:SER129 4.8 10.0 1.0 CA B:THR131 4.8 8.3 1.0 N B:LYS182 4.9 8.3 1.0 CA B:CYS178 5.0 9.6 1.0

J.Li, R.M.Wynn, M.Machius, J.L.Chuang, S.Karthikeyan, D.R.Tomchick, D.T.Chuang. Cross-Talk Between Thiamin Diphosphate Binding and Phosphorylation Loop Conformation in Human Branched-Chain Alpha-Keto Acid Decarboxylase/Dehydrogenase. J.Biol.Chem. V. 279 32968 2004.
ISSN: ISSN 0021-9258
PubMed: 15166214
DOI: 10.1074/JBC.M403611200