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Potassium in PDB 1t86: Crystal Structure of the Ferrous Cytochrome P450CAM Mutant (L358P/C334A)

Enzymatic activity of Crystal Structure of the Ferrous Cytochrome P450CAM Mutant (L358P/C334A)

All present enzymatic activity of Crystal Structure of the Ferrous Cytochrome P450CAM Mutant (L358P/C334A):
1.14.15.1;

Protein crystallography data

The structure of Crystal Structure of the Ferrous Cytochrome P450CAM Mutant (L358P/C334A), PDB code: 1t86 was solved by S.Nagano, T.Tosha, K.Ishimori, I.Morishima, T.L.Poulos, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 32.65 / 1.90
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 57.010, 101.170, 72.920, 90.00, 108.30, 90.00
R / Rfree (%) 19.9 / 23.6

Other elements in 1t86:

The structure of Crystal Structure of the Ferrous Cytochrome P450CAM Mutant (L358P/C334A) also contains other interesting chemical elements:

Iron (Fe) 2 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the Crystal Structure of the Ferrous Cytochrome P450CAM Mutant (L358P/C334A) (pdb code 1t86). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of the Ferrous Cytochrome P450CAM Mutant (L358P/C334A), PDB code: 1t86:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in 1t86

Go back to Potassium Binding Sites List in 1t86
Potassium binding site 1 out of 2 in the Crystal Structure of the Ferrous Cytochrome P450CAM Mutant (L358P/C334A)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of the Ferrous Cytochrome P450CAM Mutant (L358P/C334A) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K1430

b:20.5
occ:1.00
O A:GLU84 2.8 25.9 1.0
O A:TYR96 2.9 22.5 1.0
O A:GLY93 3.0 21.6 1.0
O A:GLU94 3.0 21.6 1.0
O A:HOH1495 3.1 33.8 1.0
C A:GLU94 3.6 22.6 1.0
CA A:GLU94 3.7 22.2 1.0
C A:TYR96 3.8 21.4 1.0
C A:GLU84 4.0 23.1 1.0
C A:GLY93 4.0 22.0 1.0
N A:TYR96 4.3 23.1 1.0
N A:GLU94 4.4 21.2 1.0
CA A:TYR96 4.5 23.2 1.0
CA A:CYS85 4.5 21.5 1.0
N A:ALA95 4.6 21.8 1.0
O A:HOH1656 4.6 34.9 1.0
C A:ALA95 4.7 24.1 1.0
N A:CYS85 4.7 20.4 1.0
N A:ASP97 4.7 21.2 1.0
CA A:ASP97 4.8 22.3 1.0
CB A:TYR96 4.8 20.6 1.0
CB A:SER102 5.0 21.0 1.0
CB A:GLU94 5.0 24.3 1.0
CD A:PRO86 5.0 17.2 1.0
CA A:GLU84 5.0 23.2 1.0

Potassium binding site 2 out of 2 in 1t86

Go back to Potassium Binding Sites List in 1t86
Potassium binding site 2 out of 2 in the Crystal Structure of the Ferrous Cytochrome P450CAM Mutant (L358P/C334A)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of the Ferrous Cytochrome P450CAM Mutant (L358P/C334A) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K2430

b:28.4
occ:1.00
O B:GLU84 2.8 30.7 1.0
O B:TYR96 2.9 24.6 1.0
O B:GLY93 3.0 24.4 1.0
O B:GLU94 3.1 28.6 1.0
C B:GLU94 3.6 28.7 1.0
CA B:GLU94 3.7 28.9 1.0
C B:GLU84 3.9 30.5 1.0
C B:TYR96 4.0 26.1 1.0
C B:GLY93 4.1 27.4 1.0
N B:TYR96 4.3 26.7 1.0
N B:GLU94 4.4 28.4 1.0
CG B:GLU84 4.5 41.5 1.0
CA B:CYS85 4.5 26.8 1.0
N B:ALA95 4.5 28.5 1.0
CA B:TYR96 4.6 25.8 1.0
N B:CYS85 4.6 26.9 1.0
C B:ALA95 4.7 28.1 1.0
CB B:TYR96 4.9 25.8 1.0
CA B:GLU84 4.9 31.8 1.0
N B:ASP97 4.9 23.7 1.0
CB B:GLU94 5.0 33.8 1.0

Reference:

S.Nagano, T.Tosha, K.Ishimori, I.Morishima, T.L.Poulos. Crystal Structure of the Cytochrome P450CAM Mutant That Exhibits the Same Spectral Perturbations Induced By Putidaredoxin Binding. J.Biol.Chem. V. 279 42844 2004.
ISSN: ISSN 0021-9258
PubMed: 15269210
DOI: 10.1074/JBC.M404217200
Page generated: Mon Aug 12 05:26:00 2024

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