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Potassium in PDB 1t85: Crystal Structure of the Ferrous Co-Bound Cytochrome P450CAM Mutant (L358P/C334A)

Enzymatic activity of Crystal Structure of the Ferrous Co-Bound Cytochrome P450CAM Mutant (L358P/C334A)

All present enzymatic activity of Crystal Structure of the Ferrous Co-Bound Cytochrome P450CAM Mutant (L358P/C334A):
1.14.15.1;

Protein crystallography data

The structure of Crystal Structure of the Ferrous Co-Bound Cytochrome P450CAM Mutant (L358P/C334A), PDB code: 1t85 was solved by S.Nagano, T.Tosha, K.Ishimori, I.Morishima, T.L.Poulos, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 40.56 / 1.80
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 57.100, 59.280, 57.407, 90.00, 104.36, 90.00
R / Rfree (%) 19 / 21.8

Other elements in 1t85:

The structure of Crystal Structure of the Ferrous Co-Bound Cytochrome P450CAM Mutant (L358P/C334A) also contains other interesting chemical elements:

Iron (Fe) 1 atom

Potassium Binding Sites:

The binding sites of Potassium atom in the Crystal Structure of the Ferrous Co-Bound Cytochrome P450CAM Mutant (L358P/C334A) (pdb code 1t85). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of the Ferrous Co-Bound Cytochrome P450CAM Mutant (L358P/C334A), PDB code: 1t85:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in 1t85

Go back to Potassium Binding Sites List in 1t85
Potassium binding site 1 out of 2 in the Crystal Structure of the Ferrous Co-Bound Cytochrome P450CAM Mutant (L358P/C334A)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of the Ferrous Co-Bound Cytochrome P450CAM Mutant (L358P/C334A) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K428

b:15.5
occ:1.00
O A:GLU84 2.7 17.8 1.0
O A:TYR96 2.9 21.9 1.0
O A:GLY93 2.9 18.8 1.0
O A:GLU94 3.2 21.1 1.0
O A:HOH630 3.2 29.0 1.0
O A:HOH622 3.2 34.0 1.0
C A:GLU94 3.6 20.3 1.0
CA A:GLU94 3.8 19.3 1.0
C A:TYR96 3.8 20.1 1.0
O A:HOH579 3.8 26.6 1.0
C A:GLU84 3.8 19.7 1.0
C A:GLY93 4.0 19.2 1.0
N A:TYR96 4.1 19.7 1.0
CG A:GLU84 4.3 25.7 1.0
N A:GLU94 4.4 18.1 1.0
CA A:TYR96 4.4 19.4 1.0
CA A:CYS85 4.4 20.0 1.0
N A:ALA95 4.5 20.8 1.0
N A:CYS85 4.6 19.3 1.0
CB A:TYR96 4.6 18.4 1.0
C A:ALA95 4.7 19.7 1.0
N A:ASP97 4.7 18.4 1.0
O A:HOH453 4.7 21.3 1.0
CA A:GLU84 4.9 19.6 1.0
CA A:ASP97 4.9 18.2 1.0
CB A:CYS85 4.9 21.6 1.0

Potassium binding site 2 out of 2 in 1t85

Go back to Potassium Binding Sites List in 1t85
Potassium binding site 2 out of 2 in the Crystal Structure of the Ferrous Co-Bound Cytochrome P450CAM Mutant (L358P/C334A)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of the Ferrous Co-Bound Cytochrome P450CAM Mutant (L358P/C334A) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K429

b:19.8
occ:1.00
O A:VAL18 2.9 22.6 1.0
O A:PRO15 3.1 24.0 1.0
O A:PRO16 3.2 23.7 1.0
O A:HOH598 3.2 28.9 1.0
O A:HOH556 3.2 34.5 1.0
C A:PRO16 3.6 23.4 1.0
CD2 A:LEU14 3.7 24.6 1.0
C A:VAL18 3.7 23.4 1.0
O A:HOH463 3.8 23.7 1.0
CA A:PRO16 3.8 23.4 1.0
CG A:LEU14 4.0 23.2 1.0
C A:PRO15 4.0 22.8 1.0
N A:VAL18 4.1 22.4 1.0
N A:PRO16 4.3 23.2 1.0
CA A:VAL18 4.4 21.5 1.0
N A:HIS17 4.5 23.4 1.0
N A:PRO19 4.6 23.0 1.0
CG A:GLU20 4.6 36.4 1.0
CA A:PRO19 4.7 24.1 1.0
C A:HIS17 4.8 21.9 1.0
O A:LEU14 4.8 22.0 1.0
N A:GLU20 4.8 24.1 1.0
CB A:LEU14 4.8 25.3 1.0
CB A:VAL18 4.9 22.3 1.0

Reference:

S.Nagano, T.Tosha, K.Ishimori, I.Morishima, T.L.Poulos. Crystal Structure of the Cytochrome P450CAM Mutant That Exhibits the Same Spectral Perturbations Induced By Putidaredoxin Binding. J.Biol.Chem. V. 279 42844 2004.
ISSN: ISSN 0021-9258
PubMed: 15269210
DOI: 10.1074/JBC.M404217200
Page generated: Sun Dec 13 23:02:10 2020

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