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Potassium in PDB 1q82: Crystal Structure of Cc-Puromycin Bound to the A-Site of the 50S Ribosomal Subunit

Protein crystallography data

The structure of Crystal Structure of Cc-Puromycin Bound to the A-Site of the 50S Ribosomal Subunit, PDB code: 1q82 was solved by J.L.Hansen, T.M.Schmeing, P.B.Moore, T.A.Steitz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.00 / 2.98
Space group C 2 2 21
Cell size a, b, c (Å), α, β, γ (°) 213.163, 301.288, 575.397, 90.00, 90.00, 90.00
R / Rfree (%) 20.7 / 25.1

Other elements in 1q82:

The structure of Crystal Structure of Cc-Puromycin Bound to the A-Site of the 50S Ribosomal Subunit also contains other interesting chemical elements:

Magnesium (Mg) 117 atoms
Cadmium (Cd) 5 atoms
Chlorine (Cl) 22 atoms
Sodium (Na) 86 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the Crystal Structure of Cc-Puromycin Bound to the A-Site of the 50S Ribosomal Subunit (pdb code 1q82). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Cc-Puromycin Bound to the A-Site of the 50S Ribosomal Subunit, PDB code: 1q82:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in 1q82

Go back to Potassium Binding Sites List in 1q82
Potassium binding site 1 out of 2 in the Crystal Structure of Cc-Puromycin Bound to the A-Site of the 50S Ribosomal Subunit


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Cc-Puromycin Bound to the A-Site of the 50S Ribosomal Subunit within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K8201

b:71.3
occ:1.00
N7 A:A2488 3.9 46.1 1.0
OP2 A:C2487 4.0 44.5 1.0
O6 A:G2489 4.1 38.6 1.0
N7 A:G2489 4.1 36.7 1.0
N6 A:A2490 4.2 33.0 1.0
N6 A:A2488 4.4 52.3 1.0
O4 A:U2531 4.4 41.2 1.0
OP2 A:A2486 4.5 38.6 1.0
N1 A:A2532 4.6 35.0 1.0
C5 A:A2488 4.6 48.0 1.0
C2 A:A2532 4.7 36.0 1.0
C8 A:A2488 4.8 45.5 1.0
C6 A:G2489 4.8 38.4 1.0
C5 A:G2489 4.8 37.2 1.0
C6 A:A2488 4.9 50.5 1.0
OP2 A:A2488 4.9 39.6 1.0

Potassium binding site 2 out of 2 in 1q82

Go back to Potassium Binding Sites List in 1q82
Potassium binding site 2 out of 2 in the Crystal Structure of Cc-Puromycin Bound to the A-Site of the 50S Ribosomal Subunit


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Cc-Puromycin Bound to the A-Site of the 50S Ribosomal Subunit within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K8202

b:89.0
occ:1.00
O A:HOH8536 2.6 44.4 1.0
O A:HOH7295 2.8 49.4 1.0
O A:HOH8631 2.9 85.9 1.0
O A:HOH4425 3.2 99.2 1.0
O4 A:U172 3.2 33.5 1.0
O4 A:U163 3.3 34.1 1.0
O A:HOH8667 3.3 27.1 1.0
OP2 A:C162 3.3 21.6 1.0
CD N:ARG82 3.4 0.8 1.0
O A:HOH8843 3.7 46.6 1.0
O A:HOH7294 3.9 39.1 1.0
C4 A:U172 4.1 33.0 1.0
C4 A:U163 4.2 32.7 1.0
N3 A:U172 4.2 32.4 1.0
NE N:ARG82 4.3 0.5 1.0
OP2 A:A169 4.4 30.8 1.0
CG N:ARG82 4.4 0.1 1.0
N4 A:C171 4.4 37.3 1.0
OP1 A:A169 4.5 34.5 1.0
P A:C162 4.5 22.5 1.0
C5 A:U163 4.6 32.1 1.0
CB N:ARG82 4.7 0.8 1.0
MG A:MG8054 4.7 33.2 1.0
OP1 A:C162 4.8 23.0 1.0
O6 A:G164 4.8 26.3 1.0
P A:A169 4.9 31.7 1.0

Reference:

J.L.Hansen, T.M.Schmeing, P.B.Moore, T.A.Steitz. Structural Insights Into Peptide Bond Formation Proc.Natl.Acad.Sci.Usa V. 99 11670 2002.
ISSN: ISSN 0027-8424
PubMed: 12185246
DOI: 10.1073/PNAS.172404099
Page generated: Sun Dec 13 23:00:14 2020

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