Potassium in PDB 1q81: Crystal Structure of Minihelix with 3' Puromycin Bound to A-Site of the 50S Ribosomal Subunit.

The structure of Crystal Structure of Minihelix with 3' Puromycin Bound to A-Site of the 50S Ribosomal Subunit., PDB code: 1q81 was solved by J.L.Hansen, T.M.Schmeing, P.B.Moore, T.A.Steitz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.95
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	213.163, 301.288, 575.397, 90.00, 90.00, 90.00
R / Rfree (%)	21 / 25.9

The structure of Crystal Structure of Minihelix with 3' Puromycin Bound to A-Site of the 50S Ribosomal Subunit. also contains other interesting chemical elements:

Magnesium	(Mg)	117 atoms
Cadmium	(Cd)	5 atoms
Chlorine	(Cl)	22 atoms
Sodium	(Na)	86 atoms

The binding sites of Potassium atom in the Crystal Structure of Minihelix with 3' Puromycin Bound to A-Site of the 50S Ribosomal Subunit. (pdb code 1q81). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Minihelix with 3' Puromycin Bound to A-Site of the 50S Ribosomal Subunit., PDB code: 1q81:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of Minihelix with 3' Puromycin Bound to A-Site of the 50S Ribosomal Subunit.


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Minihelix with 3' Puromycin Bound to A-Site of the 50S Ribosomal Subunit. within 5.0Å range: probe atom residue distance (Å) B Occ A:K8201 b:53.9 occ:1.00 O6 A:G2482 2.6 33.4 1.0 O6 A:G2102 2.8 42.8 1.0 N7 A:G2482 3.1 33.4 1.0 O4' A:C2536 3.1 37.3 1.0 O2 A:C2536 3.2 34.8 1.0 C6 A:G2482 3.4 32.3 1.0 N7 A:G2102 3.4 44.1 1.0 C5 A:G2482 3.5 33.7 1.0 C1' A:C2536 3.6 35.7 1.0 C6 A:G2102 3.6 43.9 1.0 C2 A:C2536 3.7 35.5 1.0 C5 A:G2102 3.8 43.9 1.0 N1 A:C2536 3.9 36.8 1.0 C4' A:C2536 3.9 36.0 1.0 N6 A:A2486 4.1 47.4 1.0 O2 A:U2535 4.1 44.5 1.0 C8 A:G2482 4.3 33.5 1.0 OP1 A:U2539 4.4 42.0 1.0 C2' A:U2535 4.4 40.7 1.0 C2 A:U2535 4.4 43.6 1.0 C5' A:C2536 4.5 35.5 1.0 N3 A:C2536 4.6 35.0 1.0 C8 A:G2102 4.6 44.1 1.0 N1 A:G2482 4.7 31.1 1.0 O2' A:U2535 4.7 41.7 1.0 N3 A:U2535 4.8 43.6 1.0 C4 A:G2482 4.9 34.1 1.0 N1 A:G2102 4.9 45.2 1.0 N1 A:U2535 4.9 42.3 1.0 C6 A:C2536 4.9 38.3 1.0 O5' A:C2536 5.0 37.0 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of Minihelix with 3' Puromycin Bound to A-Site of the 50S Ribosomal Subunit.


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Minihelix with 3' Puromycin Bound to A-Site of the 50S Ribosomal Subunit. within 5.0Å range: probe atom residue distance (Å) B Occ A:K8202 b:43.8 occ:1.00 O A:HOH8535 2.7 17.0 1.0 O4 A:U172 2.7 42.4 1.0 O A:HOH4433 3.1 55.1 1.0 O4 A:U163 3.1 40.0 1.0 O A:HOH7304 3.1 32.7 1.0 O A:HOH8635 3.2 34.3 1.0 OP2 A:C162 3.2 30.9 1.0 O A:HOH8670 3.3 25.5 1.0 CD N:ARG82 3.7 63.0 1.0 C4 A:U172 3.7 41.6 1.0 O A:HOH8840 3.7 57.4 1.0 N3 A:U172 4.0 40.7 1.0 O A:HOH7303 4.1 17.7 1.0 C4 A:U163 4.1 39.7 1.0 NE N:ARG82 4.3 63.6 1.0 N4 A:C171 4.4 38.9 1.0 P A:C162 4.5 31.2 1.0 OP2 A:A169 4.7 36.7 1.0 C5 A:U163 4.7 39.7 1.0 MG A:MG8054 4.8 43.2 1.0 OP1 A:A169 4.8 33.2 1.0 O6 A:G164 4.9 32.7 1.0 OP1 A:C162 4.9 32.8 1.0 N3 A:C173 4.9 32.5 1.0 CG N:ARG82 4.9 62.0 1.0 CB N:ARG82 5.0 62.7 1.0

J.L.Hansen, T.M.Schmeing, P.B.Moore, T.A.Steitz. Structural Insights Into Peptide Bond Formation Proc.Natl.Acad.Sci.Usa V. 99 11670 2002.
ISSN: ISSN 0027-8424
PubMed: 12185246
DOI: 10.1073/PNAS.172404099