Potassium in PDB 1ooy: Succinyl-Coa:3-Ketoacid Coa Transferase From Pig Heart
Enzymatic activity of Succinyl-Coa:3-Ketoacid Coa Transferase From Pig Heart
All present enzymatic activity of Succinyl-Coa:3-Ketoacid Coa Transferase From Pig Heart:
2.8.3.5;
Protein crystallography data
The structure of Succinyl-Coa:3-Ketoacid Coa Transferase From Pig Heart, PDB code: 1ooy
was solved by
A.M.Coros,
L.Swenson,
W.T.Wolodko,
M.E.Fraser,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
19.81 /
1.70
|
Space group
|
P 21 21 2
|
Cell size a, b, c (Å), α, β, γ (°)
|
99.449,
140.325,
68.095,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
15.6 /
19.4
|
Potassium Binding Sites:
The binding sites of Potassium atom in the Succinyl-Coa:3-Ketoacid Coa Transferase From Pig Heart
(pdb code 1ooy). This binding sites where shown within
5.0 Angstroms radius around Potassium atom.
In total 4 binding sites of Potassium where determined in the
Succinyl-Coa:3-Ketoacid Coa Transferase From Pig Heart, PDB code: 1ooy:
Jump to Potassium binding site number:
1;
2;
3;
4;
Potassium binding site 1 out
of 4 in 1ooy
Go back to
Potassium Binding Sites List in 1ooy
Potassium binding site 1 out
of 4 in the Succinyl-Coa:3-Ketoacid Coa Transferase From Pig Heart
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 1 of Succinyl-Coa:3-Ketoacid Coa Transferase From Pig Heart within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:K993
b:16.5
occ:1.00
|
O
|
A:HOH1181
|
2.6
|
18.4
|
1.0
|
O
|
A:HOH1080
|
2.7
|
17.9
|
1.0
|
O
|
A:LYS424
|
2.7
|
12.0
|
1.0
|
O
|
A:GLN425
|
2.9
|
14.2
|
1.0
|
O
|
A:HOH1183
|
2.9
|
18.3
|
1.0
|
O
|
A:HOH1093
|
3.1
|
26.1
|
1.0
|
C
|
A:GLN425
|
3.5
|
13.1
|
1.0
|
C
|
A:LYS424
|
3.9
|
12.1
|
1.0
|
O
|
A:HOH1336
|
4.0
|
25.8
|
1.0
|
N
|
A:CYS426
|
4.1
|
12.4
|
1.0
|
CA
|
A:CYS426
|
4.1
|
13.3
|
1.0
|
CB
|
A:CYS426
|
4.2
|
13.0
|
1.0
|
CB
|
A:GLN425
|
4.3
|
14.0
|
1.0
|
CA
|
A:GLN425
|
4.4
|
12.0
|
1.0
|
O
|
A:HOH1514
|
4.4
|
12.5
|
1.0
|
O
|
A:HOH1064
|
4.5
|
16.6
|
1.0
|
N
|
A:GLN425
|
4.6
|
11.6
|
1.0
|
O
|
A:HOH1145
|
4.9
|
25.6
|
1.0
|
O
|
A:HOH1002
|
4.9
|
11.5
|
1.0
|
N
|
A:LYS424
|
5.0
|
11.4
|
1.0
|
O
|
A:VAL391
|
5.0
|
12.9
|
1.0
|
CA
|
A:LYS424
|
5.0
|
12.9
|
1.0
|
|
Potassium binding site 2 out
of 4 in 1ooy
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Potassium Binding Sites List in 1ooy
Potassium binding site 2 out
of 4 in the Succinyl-Coa:3-Ketoacid Coa Transferase From Pig Heart
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 2 of Succinyl-Coa:3-Ketoacid Coa Transferase From Pig Heart within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:K994
b:23.5
occ:1.00
|
O
|
A:HOH1145
|
2.5
|
25.6
|
1.0
|
O
|
A:THR396
|
2.5
|
13.0
|
1.0
|
O
|
A:HOH1167
|
2.7
|
19.2
|
1.0
|
OD1
|
A:ASN428
|
2.7
|
12.6
|
1.0
|
O
|
A:SER393
|
3.2
|
15.8
|
1.0
|
O
|
A:CYS426
|
3.4
|
16.2
|
1.0
|
CG
|
A:ASN428
|
3.6
|
15.8
|
1.0
|
C
|
A:THR396
|
3.7
|
10.9
|
1.0
|
O
|
A:ALA394
|
3.7
|
20.7
|
1.0
|
ND2
|
A:ASN428
|
3.7
|
15.2
|
1.0
|
CG2
|
A:VAL398
|
3.8
|
13.1
|
1.0
|
O
|
A:HOH1183
|
3.9
|
18.3
|
1.0
|
N
|
A:VAL398
|
4.1
|
10.7
|
1.0
|
C
|
A:ALA394
|
4.1
|
18.4
|
1.0
|
CA
|
A:ALA394
|
4.2
|
17.0
|
1.0
|
C
|
A:SER393
|
4.3
|
14.2
|
1.0
|
O
|
A:HOH1228
|
4.3
|
25.8
|
1.0
|
CB
|
A:VAL398
|
4.3
|
12.8
|
1.0
|
C
|
A:CYS426
|
4.4
|
14.3
|
1.0
|
CA
|
A:LYS397
|
4.4
|
11.2
|
1.0
|
N
|
A:THR396
|
4.5
|
13.1
|
1.0
|
N
|
A:LYS397
|
4.5
|
12.1
|
1.0
|
C
|
A:LYS397
|
4.5
|
11.2
|
1.0
|
O
|
A:HOH1487
|
4.6
|
38.9
|
1.0
|
CA
|
A:THR396
|
4.7
|
13.4
|
1.0
|
N
|
A:ALA394
|
4.7
|
16.0
|
1.0
|
CA
|
A:CYS426
|
4.8
|
13.3
|
1.0
|
CA
|
A:VAL398
|
4.9
|
10.6
|
1.0
|
N
|
A:LYS395
|
5.0
|
15.8
|
1.0
|
|
Potassium binding site 3 out
of 4 in 1ooy
Go back to
Potassium Binding Sites List in 1ooy
Potassium binding site 3 out
of 4 in the Succinyl-Coa:3-Ketoacid Coa Transferase From Pig Heart
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 3 of Succinyl-Coa:3-Ketoacid Coa Transferase From Pig Heart within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:K995
b:27.7
occ:1.00
|
O
|
B:THR396
|
2.5
|
17.8
|
1.0
|
O
|
B:HOH1120
|
2.6
|
28.8
|
1.0
|
OD1
|
B:ASN428
|
2.6
|
17.8
|
1.0
|
O
|
B:HOH1228
|
2.7
|
26.0
|
1.0
|
O
|
B:CYS426
|
3.3
|
18.4
|
1.0
|
O
|
B:SER393
|
3.5
|
17.3
|
1.0
|
CG
|
B:ASN428
|
3.5
|
18.6
|
1.0
|
C
|
B:THR396
|
3.7
|
17.0
|
1.0
|
CG2
|
B:VAL398
|
3.7
|
15.0
|
1.0
|
ND2
|
B:ASN428
|
3.7
|
18.1
|
1.0
|
O
|
B:ALA394
|
3.9
|
24.8
|
1.0
|
N
|
B:VAL398
|
3.9
|
14.2
|
1.0
|
O
|
B:HOH1175
|
4.0
|
24.8
|
1.0
|
CB
|
B:VAL398
|
4.2
|
14.6
|
1.0
|
C
|
B:ALA394
|
4.3
|
22.6
|
1.0
|
O
|
B:HOH1293
|
4.3
|
36.9
|
1.0
|
C
|
B:CYS426
|
4.3
|
18.3
|
1.0
|
CA
|
B:ALA394
|
4.3
|
21.3
|
1.0
|
CA
|
B:LYS397
|
4.3
|
17.1
|
1.0
|
C
|
B:LYS397
|
4.4
|
15.1
|
1.0
|
N
|
B:LYS397
|
4.4
|
16.7
|
1.0
|
C
|
B:SER393
|
4.5
|
17.9
|
1.0
|
N
|
B:THR396
|
4.6
|
19.4
|
1.0
|
CA
|
B:THR396
|
4.7
|
17.1
|
1.0
|
CA
|
B:VAL398
|
4.7
|
14.7
|
1.0
|
CA
|
B:CYS426
|
4.8
|
18.0
|
1.0
|
CB
|
B:ASN428
|
4.9
|
17.2
|
1.0
|
N
|
B:ALA394
|
5.0
|
19.4
|
1.0
|
|
Potassium binding site 4 out
of 4 in 1ooy
Go back to
Potassium Binding Sites List in 1ooy
Potassium binding site 4 out
of 4 in the Succinyl-Coa:3-Ketoacid Coa Transferase From Pig Heart
Mono view
Stereo pair view
|
A full contact list of Potassium with other atoms in the K binding
site number 4 of Succinyl-Coa:3-Ketoacid Coa Transferase From Pig Heart within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:K996
b:30.3
occ:1.00
|
O
|
B:HOH1154
|
2.5
|
25.5
|
1.0
|
O
|
B:HOH1205
|
2.7
|
27.5
|
1.0
|
O
|
B:LYS424
|
2.7
|
16.2
|
1.0
|
O
|
B:HOH1175
|
2.8
|
24.8
|
1.0
|
O
|
B:GLN425
|
2.9
|
17.6
|
1.0
|
O
|
B:HOH1244
|
3.3
|
29.3
|
1.0
|
C
|
B:GLN425
|
3.5
|
15.7
|
1.0
|
NH1
|
B:ARG441
|
3.8
|
44.1
|
1.0
|
C
|
B:LYS424
|
3.9
|
17.0
|
1.0
|
CA
|
B:CYS426
|
4.0
|
18.0
|
1.0
|
CB
|
B:CYS426
|
4.1
|
17.2
|
1.0
|
N
|
B:CYS426
|
4.1
|
17.5
|
1.0
|
O4
|
B:PO4997
|
4.2
|
57.7
|
1.0
|
O
|
B:HOH1043
|
4.4
|
17.4
|
1.0
|
CB
|
B:GLN425
|
4.4
|
17.6
|
1.0
|
CA
|
B:GLN425
|
4.4
|
17.4
|
1.0
|
O
|
B:HOH1120
|
4.6
|
28.8
|
1.0
|
N
|
B:GLN425
|
4.7
|
17.7
|
1.0
|
CZ
|
B:ARG441
|
4.7
|
43.1
|
1.0
|
O1
|
B:PO4997
|
4.7
|
56.6
|
1.0
|
O
|
B:HOH1144
|
4.8
|
25.7
|
1.0
|
O
|
B:VAL391
|
4.9
|
18.0
|
1.0
|
NH2
|
B:ARG441
|
4.9
|
44.5
|
1.0
|
N
|
B:LYS424
|
5.0
|
17.4
|
1.0
|
|
Reference:
A.M.Coros,
L.Swenson,
W.T.Wolodko,
M.E.Fraser.
Structure of the Coa Transferase From Pig Heart to 1.7 A Resolution. Acta Crystallogr.,Sect.D V. 60 1717 2004.
ISSN: ISSN 0907-4449
PubMed: 15388917
DOI: 10.1107/S0907444904017974
Page generated: Mon Aug 12 05:07:44 2024
|