Potassium in PDB 1nki: Crystal Structure of the Fosfomycin Resistance Protein A (Fosa) Containing Bound Phosphonoformate

All present enzymatic activity of Crystal Structure of the Fosfomycin Resistance Protein A (Fosa) Containing Bound Phosphonoformate:
2.5.1.18;

The structure of Crystal Structure of the Fosfomycin Resistance Protein A (Fosa) Containing Bound Phosphonoformate, PDB code: 1nki was solved by C.L.Rife, R.E.Pharris, M.E.Newcomer, R.N.Armstrong, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 0.95
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	54.982, 66.895, 76.723, 90.00, 90.00, 90.00
R / Rfree (%)	14.7 / 17.6

The structure of Crystal Structure of the Fosfomycin Resistance Protein A (Fosa) Containing Bound Phosphonoformate also contains other interesting chemical elements:

Manganese

(Mn)

2 atoms

The binding sites of Potassium atom in the Crystal Structure of the Fosfomycin Resistance Protein A (Fosa) Containing Bound Phosphonoformate (pdb code 1nki). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of the Fosfomycin Resistance Protein A (Fosa) Containing Bound Phosphonoformate, PDB code: 1nki:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of the Fosfomycin Resistance Protein A (Fosa) Containing Bound Phosphonoformate


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of the Fosfomycin Resistance Protein A (Fosa) Containing Bound Phosphonoformate within 5.0Å range: probe atom residue distance (Å) B Occ A:K3002 b:10.9 occ:1.00 OD1 A:ASN92 2.6 12.9 1.0 OG A:SER94 2.7 12.5 1.0 O A:GLY96 2.7 11.9 1.0 O A:HOH9366 2.8 10.7 1.0 OG A:SER98 2.8 10.7 1.0 O A:SER94 3.0 13.2 1.0 O A:GLU95 3.0 11.6 1.0 C A:SER94 3.4 12.9 1.0 CG A:ASN92 3.5 11.5 1.0 CB A:SER98 3.6 9.9 1.0 CE1 A:HIS112 3.6 10.4 1.0 C A:GLU95 3.7 11.9 1.0 NH2 A:ARG119 3.7 12.7 1.0 ND1 A:HIS112 3.7 9.7 1.0 OH A:TYR100 3.8 10.8 1.0 ND2 A:ASN92 3.8 17.1 1.0 CB A:SER94 3.8 13.9 1.0 C A:GLY96 3.9 11.0 1.0 CA A:SER94 3.9 13.8 1.0 N A:SER94 4.1 13.9 1.0 N A:GLU95 4.1 13.7 1.0 O A:HOH9029 4.2 13.2 1.0 N A:GLY96 4.3 11.5 1.0 N A:SER98 4.4 9.5 1.0 OE2 A:GLU110 4.4 9.4 1.0 CA A:GLY96 4.4 11.4 1.0 O2 A:PPF5001 4.5 11.8 1.0 C A:ASP97 4.5 9.6 1.0 CA A:GLU95 4.6 13.4 1.0 CA A:SER98 4.6 9.4 1.0 O A:ASP97 4.6 10.3 1.0 NE2 A:HIS112 4.8 10.1 1.0 CZ A:TYR100 4.8 9.4 1.0 CZ A:ARG119 4.9 11.9 1.0 CB A:ASN92 4.9 11.0 1.0 N A:ASP97 4.9 11.5 1.0 CG A:HIS112 5.0 9.1 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of the Fosfomycin Resistance Protein A (Fosa) Containing Bound Phosphonoformate


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of the Fosfomycin Resistance Protein A (Fosa) Containing Bound Phosphonoformate within 5.0Å range: probe atom residue distance (Å) B Occ B:K3001 b:10.6 occ:0.95 OD1 B:ASN92 2.7 12.5 1.0 OG B:SER94 2.7 11.8 1.0 O B:GLY96 2.8 12.2 1.0 O B:HOH9375 2.8 10.8 1.0 OG B:SER98 2.8 10.8 1.0 O B:SER94 2.9 13.1 1.0 O B:GLU95 2.9 11.6 1.0 C B:SER94 3.4 12.2 1.0 CG B:ASN92 3.6 12.1 1.0 CB B:SER98 3.6 10.1 1.0 C B:GLU95 3.7 11.1 1.0 CE1 B:HIS112 3.7 10.4 1.0 OH B:TYR100 3.7 10.7 1.0 NH2 B:ARG119 3.7 12.3 1.0 ND2 B:ASN92 3.8 17.5 1.0 ND1 B:HIS112 3.8 10.2 1.0 CB B:SER94 3.8 12.8 1.0 C B:GLY96 3.9 11.9 1.0 CA B:SER94 3.9 13.0 1.0 N B:SER94 4.1 13.4 1.0 N B:GLU95 4.1 12.6 1.0 O B:HOH9021 4.2 13.1 1.0 N B:GLY96 4.3 12.0 1.0 OE2 B:GLU110 4.4 9.6 1.0 N B:SER98 4.5 9.9 1.0 CA B:GLY96 4.5 12.0 1.0 O2 B:PPF5002 4.5 11.7 1.0 CA B:GLU95 4.5 11.6 1.0 C B:ASP97 4.5 10.8 1.0 CA B:SER98 4.6 9.8 1.0 O B:ASP97 4.7 10.7 1.0 CZ B:TYR100 4.8 9.5 1.0 NE2 B:HIS112 4.8 9.8 1.0 O B:HOH9357 4.9 44.1 1.0 CZ B:ARG119 4.9 10.7 1.0 CB B:ASN92 4.9 11.6 1.0 CE1 B:TYR100 5.0 9.7 1.0 N B:ASP97 5.0 12.2 1.0 O1 B:PPF5002 5.0 10.0 1.0

R.E.Rigsby, C.L.Rife, K.L.Fillgrove, M.E.Newcomer, R.N.Armstrong. Phosphonoformate: A Minimal Transition State Analogue Inhibitor of the Fosfomycin Resistance Protein, Fosa. Biochemistry V. 43 13666 2004.
ISSN: ISSN 0006-2960
PubMed: 15504029
DOI: 10.1021/BI048767H