Potassium in PDB 1mp0: Binary Complex of Human Glutathione-Dependent Formaldehyde Dehydrogenase with Nad(H)

All present enzymatic activity of Binary Complex of Human Glutathione-Dependent Formaldehyde Dehydrogenase with Nad(H):
1.1.1.1; 1.2.1.1;

The structure of Binary Complex of Human Glutathione-Dependent Formaldehyde Dehydrogenase with Nad(H), PDB code: 1mp0 was solved by P.C.Sanghani, H.Robinson, T.D.Hurley, W.F.Bosron, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.90 / 2.20
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	79.033, 79.033, 311.755, 90.00, 90.00, 90.00
R / Rfree (%)	18.9 / 21.8

The structure of Binary Complex of Human Glutathione-Dependent Formaldehyde Dehydrogenase with Nad(H) also contains other interesting chemical elements:

Zinc

(Zn)

4 atoms

The binding sites of Potassium atom in the Binary Complex of Human Glutathione-Dependent Formaldehyde Dehydrogenase with Nad(H) (pdb code 1mp0). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Binary Complex of Human Glutathione-Dependent Formaldehyde Dehydrogenase with Nad(H), PDB code: 1mp0:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Binary Complex of Human Glutathione-Dependent Formaldehyde Dehydrogenase with Nad(H)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Binary Complex of Human Glutathione-Dependent Formaldehyde Dehydrogenase with Nad(H) within 5.0Å range: probe atom residue distance (Å) B Occ A:K701 b:20.7 occ:1.00 OH A:TYR263 2.8 17.2 1.0 OE2 A:GLU189 2.8 17.2 1.0 O A:HOH985 2.9 18.3 1.0 O A:HOH1087 2.9 41.5 1.0 O A:LYS187 2.9 21.6 1.0 O A:ALA186 3.0 17.4 1.0 O B:HOH1052 3.2 35.6 1.0 C A:LYS187 3.7 20.7 1.0 CD A:GLU189 3.7 17.6 1.0 CZ A:TYR263 3.8 17.5 1.0 O A:HOH845 3.9 17.6 1.0 CB A:LYS187 3.9 23.6 1.0 OE1 A:GLU189 4.0 15.8 1.0 CE2 A:TYR263 4.1 16.2 1.0 C A:ALA186 4.2 18.9 1.0 CA A:LYS187 4.3 21.6 1.0 NZ B:LYS106 4.3 18.1 1.0 O B:HOH847 4.4 23.3 1.0 N A:LEU188 4.4 20.1 1.0 O A:HOH1053 4.4 35.2 1.0 CA A:LEU188 4.6 19.9 1.0 N A:LYS187 4.7 20.1 1.0 O A:HOH930 4.7 25.6 1.0 CG A:GLU189 4.8 15.1 1.0

Potassium binding site 2 out of 2 in the Binary Complex of Human Glutathione-Dependent Formaldehyde Dehydrogenase with Nad(H)


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Binary Complex of Human Glutathione-Dependent Formaldehyde Dehydrogenase with Nad(H) within 5.0Å range: probe atom residue distance (Å) B Occ B:K702 b:25.2 occ:1.00 OH B:TYR263 2.7 14.2 1.0 O B:LYS187 2.8 21.0 1.0 OE2 B:GLU189 2.9 24.9 1.0 O B:HOH1032 2.9 30.9 1.0 O B:HOH1000 2.9 24.1 1.0 O B:ALA186 3.0 18.9 1.0 O A:HOH995 3.0 18.3 1.0 O B:HOH1045 3.4 46.7 1.0 C B:LYS187 3.6 21.2 1.0 CD B:GLU189 3.7 23.9 1.0 CZ B:TYR263 3.8 15.7 1.0 OE1 B:GLU189 4.0 21.1 1.0 CB B:LYS187 4.0 23.2 1.0 O B:HOH817 4.0 18.1 1.0 CE2 B:TYR263 4.1 13.2 1.0 C B:ALA186 4.1 19.6 1.0 NZ A:LYS106 4.3 16.9 1.0 CA B:LYS187 4.3 22.0 1.0 N B:LEU188 4.4 20.0 1.0 O A:HOH879 4.4 16.5 1.0 CA B:LEU188 4.5 19.3 1.0 N B:LYS187 4.7 19.8 1.0 O B:HOH937 4.8 32.0 1.0 CG B:GLU189 4.8 21.6 1.0 CE1 B:TYR263 4.9 14.9 1.0

P.C.Sanghani, H.Robinson, R.Bennett-Lovsey, T.D.Hurley, W.F.Bosron. Structure-Function Relationships in Human Class III Alcohol Dehydrogenase (Formaldehyde Dehydrogenase) Chem.Biol.Interact. V. 143 195 2003.
ISSN: ISSN 0009-2797
PubMed: 12604204
DOI: 10.1016/S0009-2797(02)00203-X