Potassium in PDB 1fp7: Monovalent Cation Binding Sites in N10-Formyltetrahydrofolate Synthetase From Moorella Thermoacetica

All present enzymatic activity of Monovalent Cation Binding Sites in N10-Formyltetrahydrofolate Synthetase From Moorella Thermoacetica:
6.3.4.3;

The structure of Monovalent Cation Binding Sites in N10-Formyltetrahydrofolate Synthetase From Moorella Thermoacetica, PDB code: 1fp7 was solved by R.Radfar, A.Leaphart, J.M.Brewer, W.Minor, J.D.Odom, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.99 / 3.20
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	160.876, 160.876, 256.118, 90.00, 90.00, 120.00
R / Rfree (%)	28.5 / 35.5

The binding sites of Potassium atom in the Monovalent Cation Binding Sites in N10-Formyltetrahydrofolate Synthetase From Moorella Thermoacetica (pdb code 1fp7). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Monovalent Cation Binding Sites in N10-Formyltetrahydrofolate Synthetase From Moorella Thermoacetica, PDB code: 1fp7:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Monovalent Cation Binding Sites in N10-Formyltetrahydrofolate Synthetase From Moorella Thermoacetica


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Monovalent Cation Binding Sites in N10-Formyltetrahydrofolate Synthetase From Moorella Thermoacetica within 5.0Å range: probe atom residue distance (Å) B Occ A:K282 b:38.9 occ:0.50 O A:HOH43 1.7 16.3 1.0 OD1 A:ASP1132 1.8 17.2 1.0 OE1 A:GLU1098 2.0 25.7 1.0 CG A:ASP1132 2.3 20.8 1.0 O A:HOH270 2.3 0.0 1.0 CB A:ASP1132 2.4 21.6 1.0 CD A:GLU1098 3.2 22.4 1.0 ND2 A:ASN1258 3.2 24.6 1.0 OD2 A:ASP1132 3.4 18.8 1.0 OG1 A:THR1130 3.6 22.1 1.0 CA A:ASP1132 3.7 21.7 1.0 CD2 A:PHE1129 3.8 28.8 1.0 N A:ASP1132 3.8 22.7 1.0 OE2 A:GLU1098 4.0 21.2 1.0 CG A:GLU1098 4.1 21.4 1.0 CG A:ASN1258 4.2 28.3 1.0 CB A:GLU1098 4.3 19.8 1.0 CE2 A:PHE1129 4.4 28.9 1.0 O A:HOH186 4.6 17.9 1.0 OD1 A:ASN1258 4.6 32.3 1.0 C A:ASP1132 4.6 21.6 1.0 N A:ILE1133 4.7 19.9 1.0 N A:THR1130 4.8 20.3 1.0 CG A:PHE1129 4.8 26.8 1.0 O A:ILE1270 4.8 26.6 1.0 CB A:PHE1129 4.9 23.8 1.0 CB A:THR1130 4.9 22.3 1.0 N A:GLY1272 5.0 23.8 1.0

Potassium binding site 2 out of 2 in the Monovalent Cation Binding Sites in N10-Formyltetrahydrofolate Synthetase From Moorella Thermoacetica


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Monovalent Cation Binding Sites in N10-Formyltetrahydrofolate Synthetase From Moorella Thermoacetica within 5.0Å range: probe atom residue distance (Å) B Occ A:K282 b:49.0 occ:0.50 O A:HOH186 0.8 17.9 1.0 OE2 A:GLU1098 2.9 21.2 1.0 CB A:ILE1133 3.0 18.8 1.0 CG1 A:ILE1133 3.0 16.1 1.0 N A:ILE1133 3.2 19.9 1.0 CD1 A:ILE1133 3.4 16.8 1.0 CA A:ILE1133 3.6 20.6 1.0 O A:HIS1128 3.7 19.2 1.0 CD A:GLU1098 3.8 22.4 1.0 O A:LEU1127 4.0 26.0 1.0 N A:ASP1132 4.1 22.7 1.0 O A:HOH270 4.1 0.0 1.0 OE1 A:GLU1098 4.1 25.7 1.0 C A:HIS1128 4.2 21.9 1.0 CG2 A:ILE1133 4.3 17.4 1.0 C A:ASP1132 4.3 21.6 1.0 N A:HIS1134 4.4 23.0 1.0 C A:ILE1133 4.4 22.5 1.0 CA A:HIS1128 4.5 21.5 1.0 C A:GLY1131 4.5 21.8 1.0 CA A:GLY1131 4.6 21.5 1.0 CE1 A:HIS1134 4.6 17.2 1.0 CA A:ASP1132 4.6 21.7 1.0 ND1 A:HIS1134 4.7 16.6 1.0 N A:GLY1131 4.7 19.5 1.0 NE2 A:HIS1134 4.9 21.5 1.0 CB A:ASP1132 4.9 21.6 1.0 CB A:PHE1129 5.0 23.8 1.0

R.Radfar, A.Leaphart, J.M.Brewer, W.Minor, J.D.Odom, R.B.Dunlap, C.R.Lovell, L.Lebioda. Cation Binding and Thermostability of Fthfs Monovalent Cation Binding Sites and Thermostability of N10-Formyltetrahydrofolate Synthetase From Moorella Thermoacetica. Biochemistry V. 39 14481 2000.
ISSN: ISSN 0006-2960
PubMed: 11087401
DOI: 10.1021/BI001577W