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Potassium in PDB 1ehy: X-Ray Structure of the Epoxide Hydrolase From Agrobacterium Radiobacter AD1

Enzymatic activity of X-Ray Structure of the Epoxide Hydrolase From Agrobacterium Radiobacter AD1

All present enzymatic activity of X-Ray Structure of the Epoxide Hydrolase From Agrobacterium Radiobacter AD1:
3.3.2.10;

Protein crystallography data

The structure of X-Ray Structure of the Epoxide Hydrolase From Agrobacterium Radiobacter AD1, PDB code: 1ehy was solved by M.Nardini, I.S.Ridder, H.J.Rozeboom, K.H.Kalk, R.Rink, D.B.Janssen, B.W.Dijkstra, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 25.00 / 2.10
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 146.624, 100.201, 96.879, 90.00, 100.68, 90.00
R / Rfree (%) 19 / 22.7

Potassium Binding Sites:

The binding sites of Potassium atom in the X-Ray Structure of the Epoxide Hydrolase From Agrobacterium Radiobacter AD1 (pdb code 1ehy). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 4 binding sites of Potassium where determined in the X-Ray Structure of the Epoxide Hydrolase From Agrobacterium Radiobacter AD1, PDB code: 1ehy:
Jump to Potassium binding site number: 1; 2; 3; 4;

Potassium binding site 1 out of 4 in 1ehy

Go back to Potassium Binding Sites List in 1ehy
Potassium binding site 1 out of 4 in the X-Ray Structure of the Epoxide Hydrolase From Agrobacterium Radiobacter AD1


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of X-Ray Structure of the Epoxide Hydrolase From Agrobacterium Radiobacter AD1 within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K295

b:36.6
occ:1.00
O A:SER184 2.4 22.9 1.0
O A:HOH411 2.6 31.2 1.0
O A:ARG186 2.6 36.4 1.0
O A:ASP181 2.8 30.1 1.0
O A:HOH352 2.9 41.6 1.0
C A:SER184 3.5 30.0 1.0
O A:TYR185 3.5 37.2 1.0
C A:ARG186 3.6 33.6 1.0
C A:ASP181 3.8 27.4 1.0
C A:TYR185 3.9 35.7 1.0
CA A:ASP187 4.1 33.1 1.0
N A:SER184 4.2 28.5 1.0
N A:ASP187 4.2 33.1 1.0
CA A:SER184 4.2 29.2 1.0
O A:HIS182 4.3 25.4 1.0
CB A:SER184 4.4 25.2 1.0
CA A:HIS182 4.4 25.5 1.0
N A:ARG186 4.4 35.3 1.0
O A:HOH392 4.4 49.0 1.0
N A:TYR185 4.4 30.9 1.0
O A:ASP187 4.4 34.3 1.0
C A:HIS182 4.4 24.5 1.0
C A:ASP187 4.5 33.8 1.0
N A:HIS182 4.5 26.5 1.0
CA A:TYR185 4.5 34.1 1.0
CA A:ARG186 4.6 34.4 1.0
CB A:ASP246 4.7 71.9 1.0
CA A:ASP181 4.8 28.3 1.0
OD1 A:ASP187 4.8 38.1 1.0

Potassium binding site 2 out of 4 in 1ehy

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Potassium binding site 2 out of 4 in the X-Ray Structure of the Epoxide Hydrolase From Agrobacterium Radiobacter AD1


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of X-Ray Structure of the Epoxide Hydrolase From Agrobacterium Radiobacter AD1 within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K296

b:29.9
occ:1.00
O B:ASP181 2.6 27.5 1.0
O B:ARG186 2.7 31.8 1.0
O B:SER184 2.7 25.4 1.0
O B:HOH359 2.9 54.7 1.0
O B:HOH440 3.0 31.1 1.0
C B:SER184 3.6 23.4 1.0
C B:ARG186 3.6 31.6 1.0
C B:ASP181 3.7 26.7 1.0
O B:TYR185 3.8 33.8 1.0
C B:TYR185 4.0 30.7 1.0
O B:ASP187 4.2 33.8 1.0
CA B:ASP187 4.3 33.2 1.0
N B:ARG186 4.3 31.4 1.0
O B:HIS182 4.3 25.5 1.0
N B:ASP187 4.4 30.9 1.0
O B:HOH401 4.4 47.0 1.0
N B:SER184 4.4 20.7 1.0
CA B:SER184 4.4 21.2 1.0
CA B:HIS182 4.4 26.6 1.0
N B:TYR185 4.4 25.3 1.0
N B:HIS182 4.4 25.4 1.0
C B:HIS182 4.5 24.1 1.0
CA B:TYR185 4.5 28.7 1.0
C B:ASP187 4.5 33.7 1.0
CB B:SER184 4.5 21.2 1.0
CA B:ARG186 4.6 31.1 1.0
CA B:ASP181 4.6 25.0 1.0
CB B:ASP181 5.0 27.9 1.0

Potassium binding site 3 out of 4 in 1ehy

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Potassium binding site 3 out of 4 in the X-Ray Structure of the Epoxide Hydrolase From Agrobacterium Radiobacter AD1


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of X-Ray Structure of the Epoxide Hydrolase From Agrobacterium Radiobacter AD1 within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K297

b:33.3
occ:1.00
O C:ARG186 2.6 32.7 1.0
O C:ASP181 2.6 26.6 1.0
O C:HOH559 2.8 36.0 1.0
O C:SER184 2.9 26.7 1.0
O C:TYR185 3.6 27.8 1.0
C C:ARG186 3.6 31.9 1.0
C C:ASP181 3.6 26.5 1.0
C C:SER184 3.7 26.1 1.0
C C:TYR185 4.0 29.2 1.0
O C:ASP187 4.2 35.5 1.0
CA C:ASP187 4.2 35.4 1.0
CA C:HIS182 4.3 26.2 1.0
N C:ASP187 4.3 33.9 1.0
N C:SER184 4.3 21.6 1.0
O C:HIS182 4.4 29.6 1.0
N C:HIS182 4.4 24.5 1.0
C C:HIS182 4.4 25.9 1.0
CA C:SER184 4.4 23.8 1.0
O C:HOH601 4.4 44.9 1.0
C C:ASP187 4.4 34.0 1.0
N C:ARG186 4.5 28.7 1.0
N C:TYR185 4.5 27.6 1.0
CB C:SER184 4.6 21.2 1.0
CA C:ASP181 4.6 28.1 1.0
CA C:TYR185 4.6 29.0 1.0
CA C:ARG186 4.6 28.7 1.0

Potassium binding site 4 out of 4 in 1ehy

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Potassium binding site 4 out of 4 in the X-Ray Structure of the Epoxide Hydrolase From Agrobacterium Radiobacter AD1


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of X-Ray Structure of the Epoxide Hydrolase From Agrobacterium Radiobacter AD1 within 5.0Å range:
probe atom residue distance (Å) B Occ
D:K298

b:33.5
occ:1.00
O D:SER184 2.6 28.4 1.0
O D:ASP181 2.6 24.5 1.0
O D:ARG186 2.7 28.2 1.0
O D:TYR185 3.4 29.4 1.0
C D:SER184 3.5 24.9 1.0
C D:ASP181 3.7 23.0 1.0
C D:ARG186 3.7 28.2 1.0
C D:TYR185 3.9 29.1 1.0
O D:ASP187 4.2 29.4 1.0
CA D:ASP187 4.2 30.8 1.0
N D:SER184 4.3 21.1 1.0
CA D:SER184 4.3 22.6 1.0
N D:TYR185 4.3 25.3 1.0
N D:ASP187 4.4 28.9 1.0
CA D:HIS182 4.4 25.8 1.0
O D:HIS182 4.4 25.9 1.0
O D:HOH382 4.4 67.0 1.0
N D:HIS182 4.4 24.1 1.0
C D:HIS182 4.4 26.0 1.0
CA D:TYR185 4.5 30.9 1.0
C D:ASP187 4.5 30.1 1.0
N D:ARG186 4.5 27.9 1.0
CB D:SER184 4.5 21.2 1.0
CA D:ASP181 4.7 24.0 1.0
CA D:ARG186 4.7 27.3 1.0

Reference:

M.Nardini, I.S.Ridder, H.J.Rozeboom, K.H.Kalk, R.Rink, D.B.Janssen, B.W.Dijkstra. The X-Ray Structure of Epoxide Hydrolase From Agrobacterium Radiobacter AD1. An Enzyme to Detoxify Harmful Epoxides. J.Biol.Chem. V. 274 14579 1999.
ISSN: ISSN 0021-9258
PubMed: 10329649
DOI: 10.1074/JBC.274.21.14579
Page generated: Sun Dec 13 22:42:44 2020

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