Potassium in PDB 1egm: Crystal Structure of Diol Dehydratase-Cyanocobalamin Complex at 100K.

All present enzymatic activity of Crystal Structure of Diol Dehydratase-Cyanocobalamin Complex at 100K.:
4.2.1.28;

The structure of Crystal Structure of Diol Dehydratase-Cyanocobalamin Complex at 100K., PDB code: 1egm was solved by J.Masuda, N.Shibata, T.Toraya, Y.Morimoto, N.Yasuoka, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.85
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	74.000, 121.600, 207.700, 90.00, 90.00, 90.00
R / Rfree (%)	18.2 / 24.9

The structure of Crystal Structure of Diol Dehydratase-Cyanocobalamin Complex at 100K. also contains other interesting chemical elements:

Cobalt

(Co)

2 atoms

The binding sites of Potassium atom in the Crystal Structure of Diol Dehydratase-Cyanocobalamin Complex at 100K. (pdb code 1egm). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Diol Dehydratase-Cyanocobalamin Complex at 100K., PDB code: 1egm:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of Diol Dehydratase-Cyanocobalamin Complex at 100K.


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Diol Dehydratase-Cyanocobalamin Complex at 100K. within 5.0Å range: probe atom residue distance (Å) B Occ A:K603 b:13.7 occ:1.00 OE1 A:GLU221 2.2 15.6 1.0 OE1 A:GLU170 2.3 16.9 1.0 OE1 A:GLN296 2.3 11.4 1.0 O A:SER362 2.4 12.8 1.0 O1 A:PGO602 2.4 11.2 1.0 O2 A:PGO602 2.4 14.9 1.0 OE1 A:GLN141 2.4 16.6 1.0 C1 A:PGO602 3.2 13.8 1.0 C2 A:PGO602 3.2 17.5 1.0 CD A:GLU170 3.3 17.0 1.0 CD A:GLU221 3.3 11.8 1.0 CD A:GLN296 3.3 11.5 1.0 C A:SER362 3.4 14.4 1.0 CD A:GLN141 3.6 17.1 1.0 OE2 A:GLU170 3.6 20.3 1.0 NE2 A:GLN296 3.6 17.0 1.0 OE2 A:GLU221 3.8 16.1 1.0 CB A:SER362 3.9 13.7 1.0 NE2 A:GLN141 4.0 8.9 1.0 NE2 A:HIS143 4.0 14.8 1.0 CA A:SER362 4.2 9.7 1.0 OG A:SER362 4.2 15.4 1.0 NE2 A:GLN200 4.2 13.5 1.0 OD2 A:ASP335 4.3 14.4 1.0 N A:GLY363 4.4 17.2 1.0 CD2 A:HIS143 4.4 16.4 1.0 CG A:GLU221 4.5 8.5 1.0 CA A:GLY363 4.6 8.9 1.0 CG A:GLU170 4.6 15.0 1.0 C3 A:PGO602 4.6 11.4 1.0 CG A:GLN296 4.7 8.5 1.0 CB A:GLU170 4.7 15.7 1.0 CG A:GLN141 4.9 18.0 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of Diol Dehydratase-Cyanocobalamin Complex at 100K.


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Diol Dehydratase-Cyanocobalamin Complex at 100K. within 5.0Å range: probe atom residue distance (Å) B Occ L:K603 b:20.5 occ:1.00 OE1 L:GLU221 2.2 25.8 1.0 OE1 L:GLN141 2.3 18.6 1.0 O2 L:PGO602 2.3 24.3 1.0 O1 L:PGO602 2.3 24.6 1.0 OE1 L:GLU170 2.3 23.8 1.0 O L:SER362 2.4 17.7 1.0 OE1 L:GLN296 2.4 21.8 1.0 C2 L:PGO602 3.2 26.2 1.0 C1 L:PGO602 3.3 24.4 1.0 CD L:GLU221 3.3 24.2 1.0 CD L:GLU170 3.3 21.0 1.0 C L:SER362 3.4 24.3 1.0 CD L:GLN296 3.4 20.6 1.0 CD L:GLN141 3.4 17.2 1.0 OE2 L:GLU170 3.7 21.3 1.0 NE2 L:GLN296 3.7 18.9 1.0 OE2 L:GLU221 3.7 19.4 1.0 CB L:SER362 4.0 12.9 1.0 NE2 L:GLN141 4.0 25.0 1.0 NE2 L:HIS143 4.0 17.8 1.0 OG L:SER362 4.1 16.9 1.0 CA L:SER362 4.2 15.3 1.0 OD2 L:ASP335 4.2 22.3 1.0 NE2 L:GLN200 4.3 24.5 1.0 N L:GLY363 4.3 18.7 1.0 CA L:GLY363 4.5 17.6 1.0 CG L:GLU221 4.5 17.6 1.0 C3 L:PGO602 4.5 29.1 1.0 CD2 L:HIS143 4.5 16.6 1.0 CG L:GLU170 4.6 24.3 1.0 CG L:GLN141 4.7 21.0 1.0 CG L:GLN296 4.7 12.4 1.0 CB L:GLU170 4.7 17.7 1.0

J.Masuda, N.Shibata, Y.Morimoto, T.Toraya, N.Yasuoka. How A Protein Generates A Catalytic Radical From Coenzyme B(12): X-Ray Structure of A Diol-Dehydratase-Adeninylpentylcobalamin Complex. Structure Fold.Des. V. 8 775 2000.
ISSN: ISSN 0969-2126
PubMed: 10903944
DOI: 10.1016/S0969-2126(00)00164-7