Atomistry » Potassium » PDB 1a3w-1d7u » 1aqf
Atomistry »
  Potassium »
    PDB 1a3w-1d7u »
      1aqf »

Potassium in PDB 1aqf: Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate

Enzymatic activity of Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate

All present enzymatic activity of Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate:
2.7.1.40;

Protein crystallography data

The structure of Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate, PDB code: 1aqf was solved by T.M.Larsen, M.M.Benning, G.E.Wesenberg, I.Rayment, G.H.Reed, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 30.00 / 2.70
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 144.400, 112.600, 171.200, 90.00, 93.70, 90.00
R / Rfree (%) n/a / n/a

Other elements in 1aqf:

The structure of Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate also contains other interesting chemical elements:

Magnesium (Mg) 8 atoms

Potassium Binding Sites:

The binding sites of Potassium atom in the Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate (pdb code 1aqf). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 8 binding sites of Potassium where determined in the Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate, PDB code: 1aqf:
Jump to Potassium binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Potassium binding site 1 out of 8 in 1aqf

Go back to Potassium Binding Sites List in 1aqf
Potassium binding site 1 out of 8 in the Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K533

b:30.5
occ:1.00
 OG A:SER76 2.3 73.4 1.0
O A:THR113 2.6 75.8 1.0
OD1 A:ASN74 2.7 11.4 1.0
O2P A:PEQ532 2.8 76.5 1.0
OD1 A:ASP112 3.2 23.5 1.0
OG A:SER242 3.7 0.0 1.0
C A:THR113 3.7 19.7 1.0
CB A:SER76 3.8 19.6 1.0
CG A:ASN74 3.8 27.3 1.0
O A:HOH6014 4.0 52.0 1.0
OE2 A:GLU117 4.0 83.5 1.0
CG A:ASP112 4.1 42.1 1.0
ND2 A:ASN74 4.2 13.5 1.0
CA A:LYS114 4.2 28.9 1.0
NZ A:LYS269 4.3 3.1 1.0
P A:PEQ532 4.3 87.8 1.0
N A:SER76 4.3 32.3 1.0
N A:LYS114 4.4 29.2 1.0
NH2 A:ARG72 4.4 35.2 1.0
O A:ASP112 4.5 52.7 1.0
CA A:SER76 4.5 18.1 1.0
C A:ASP112 4.6 15.3 1.0
O2 A:PEQ532 4.6 75.9 1.0
OD2 A:ASP112 4.7 25.4 1.0
CB A:SER242 4.7 1.0 1.0
N A:THR113 4.8 3.9 1.0
CB A:ASP112 4.8 14.6 1.0
CA A:THR113 4.8 26.9 1.0
O1P A:PEQ532 4.9 79.1 1.0
N A:PHE75 5.0 23.2 1.0

Potassium binding site 2 out of 8 in 1aqf

Go back to Potassium Binding Sites List in 1aqf
Potassium binding site 2 out of 8 in the Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K533

b:73.4
occ:1.00
 OG B:SER76 2.5 0.0 1.0
O2P B:PEQ532 2.7 49.0 0.5
OD1 B:ASN74 2.8 58.8 1.0
O B:THR113 3.1 40.1 1.0
OD1 B:ASP112 3.1 60.0 1.0
OG B:SER242 3.7 13.6 1.0
NZ B:LYS269 3.7 6.7 1.0
C B:THR113 3.9 37.2 1.0
CB B:SER76 3.9 18.1 1.0
CG B:ASN74 3.9 24.5 1.0
CA B:LYS114 4.0 28.9 1.0
CG B:ASP112 4.1 12.4 1.0
O B:ASP112 4.2 42.1 1.0
P B:PEQ532 4.2 98.1 0.5
N B:LYS114 4.3 55.0 1.0
ND2 B:ASN74 4.3 57.2 1.0
O B:LYS114 4.4 30.4 1.0
NH2 B:ARG72 4.5 19.6 1.0
C B:ASP112 4.5 17.8 1.0
O2 B:PEQ532 4.6 34.9 0.5
C B:LYS114 4.6 19.9 1.0
N B:SER76 4.6 92.8 1.0
OD2 B:ASP112 4.7 45.3 1.0
CA B:SER76 4.7 37.2 1.0
CB B:ASP112 4.8 29.6 1.0
CB B:SER242 4.8 21.8 1.0
O3P B:PEQ532 4.8 77.5 0.5
N B:THR113 4.9 24.3 1.0

Potassium binding site 3 out of 8 in 1aqf

Go back to Potassium Binding Sites List in 1aqf
Potassium binding site 3 out of 8 in the Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate within 5.0Å range:
probe atom residue distance (Å) B Occ
C:K533

b:75.9
occ:1.00
 OG C:SER76 2.8 0.0 1.0
O2P C:PEQ532 2.8 57.3 0.5
O C:THR113 2.9 41.0 1.0
OD1 C:ASN74 3.1 33.1 1.0
OD1 C:ASP112 3.2 29.8 1.0
C C:THR113 3.5 23.1 1.0
O C:ASP112 3.7 50.6 1.0
CA C:LYS114 3.7 27.2 1.0
N C:LYS114 3.9 53.7 1.0
NZ C:LYS269 3.9 16.2 1.0
OG C:SER242 4.0 14.2 1.0
CG C:ASN74 4.0 16.8 1.0
CB C:SER76 4.1 9.3 1.0
CG C:ASP112 4.2 20.8 1.0
C C:ASP112 4.2 27.4 1.0
P C:PEQ532 4.3 89.1 0.5
ND2 C:ASN74 4.4 66.9 1.0
N C:SER76 4.5 13.6 1.0
N C:THR113 4.6 8.2 1.0
C C:LYS114 4.6 30.6 1.0
CA C:THR113 4.6 14.0 1.0
CA C:SER76 4.6 25.1 1.0
CB C:LYS114 4.7 66.3 1.0
O C:LYS114 4.7 32.8 1.0
CB C:ASP112 4.8 26.1 1.0
O2 C:PEQ532 4.8 28.4 0.5
NH2 C:ARG72 4.8 23.2 1.0
O1P C:PEQ532 4.9 35.1 0.5
OD2 C:ASP112 4.9 40.1 1.0

Potassium binding site 4 out of 8 in 1aqf

Go back to Potassium Binding Sites List in 1aqf
Potassium binding site 4 out of 8 in the Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate within 5.0Å range:
probe atom residue distance (Å) B Occ
D:K533

b:70.4
occ:1.00
 O D:THR113 2.5 28.1 1.0
O2P D:PEQ532 2.6 19.1 0.5
OD1 D:ASP112 3.1 13.8 1.0
OD1 D:ASN74 3.2 40.7 1.0
C D:THR113 3.4 31.2 1.0
OG D:SER76 3.5 64.9 1.0
NZ D:LYS269 3.7 3.5 1.0
OG D:SER242 3.8 37.7 1.0
CA D:LYS114 3.9 30.4 1.0
CG D:ASP112 4.0 71.5 1.0
N D:LYS114 4.0 37.9 1.0
O D:ASP112 4.0 53.9 1.0
P D:PEQ532 4.1 68.0 0.5
CG D:ASN74 4.1 1.4 1.0
C D:ASP112 4.2 29.3 1.0
CB D:SER76 4.2 16.2 1.0
O D:LYS114 4.3 41.9 1.0
N D:THR113 4.3 27.3 1.0
C D:LYS114 4.3 34.4 1.0
CA D:THR113 4.4 17.9 1.0
N D:SER76 4.4 16.9 1.0
OD2 D:ASP112 4.6 18.0 1.0
ND2 D:ASN74 4.6 40.4 1.0
CA D:SER76 4.6 30.1 1.0
O1P D:PEQ532 4.6 69.8 0.5
CB D:ASP112 4.7 9.8 1.0
O2 D:PEQ532 4.8 62.4 0.5
CA D:ASP112 5.0 12.3 1.0
CB D:SER242 5.0 12.9 1.0

Potassium binding site 5 out of 8 in 1aqf

Go back to Potassium Binding Sites List in 1aqf
Potassium binding site 5 out of 8 in the Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 5 of Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate within 5.0Å range:
probe atom residue distance (Å) B Occ
E:K533

b:81.8
occ:1.00
 OG E:SER76 2.7 0.0 1.0
OD1 E:ASP112 2.8 72.8 1.0
O2P E:PEQ532 2.8 0.0 0.5
O E:THR113 3.0 44.3 1.0
OD1 E:ASN74 3.1 63.5 1.0
CB E:SER76 3.6 89.4 1.0
CA E:LYS114 3.8 74.4 1.0
C E:THR113 3.9 73.3 1.0
CG E:ASN74 3.9 32.4 1.0
ND2 E:ASN74 4.0 69.3 1.0
P E:PEQ532 4.0 82.4 0.5
CG E:ASP112 4.0 76.6 1.0
OG E:SER242 4.0 0.0 1.0
O1P E:PEQ532 4.0 22.8 0.5
NZ E:LYS269 4.1 30.5 1.0
MG E:MG534 4.1 82.0 1.0
N E:LYS114 4.2 46.4 1.0
N E:SER76 4.3 49.0 1.0
CA E:SER76 4.4 18.8 1.0
O E:LYS114 4.4 72.7 1.0
OD2 E:ASP112 4.5 32.4 1.0
C E:LYS114 4.5 49.2 1.0
O2 E:PEQ532 4.6 86.3 0.5
CB E:LYS114 4.7 45.6 1.0
NH2 E:ARG72 4.8 21.2 1.0
C E:ASP112 4.9 29.6 1.0
N E:THR113 4.9 72.3 1.0
CB E:ASP112 5.0 19.1 1.0
O E:ASP112 5.0 57.6 1.0
CA E:THR113 5.0 31.4 1.0

Potassium binding site 6 out of 8 in 1aqf

Go back to Potassium Binding Sites List in 1aqf
Potassium binding site 6 out of 8 in the Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 6 of Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate within 5.0Å range:
probe atom residue distance (Å) B Occ
F:K533

b:93.7
occ:1.00
 O F:THR113 2.5 35.6 1.0
OG F:SER76 3.0 0.0 1.0
O2P F:PEQ532 3.1 30.0 0.5
OD1 F:ASP112 3.2 15.0 1.0
OG F:SER242 3.2 98.7 1.0
C F:THR113 3.3 33.0 1.0
OD1 F:ASN74 3.3 38.5 1.0
O F:ASP112 3.5 37.3 1.0
CA F:LYS114 3.5 40.3 1.0
O F:LYS114 3.8 38.4 1.0
N F:LYS114 3.8 30.2 1.0
C F:LYS114 4.0 45.0 1.0
CG F:ASP112 4.0 73.7 1.0
C F:ASP112 4.1 36.2 1.0
NZ F:LYS269 4.1 30.9 1.0
CB F:SER242 4.2 1.0 1.0
CB F:SER76 4.3 1.4 1.0
CG F:ASN74 4.4 10.4 1.0
N F:SER76 4.4 27.1 1.0
CA F:THR113 4.5 36.0 1.0
N F:THR113 4.5 40.3 1.0
CB F:LYS114 4.5 0.0 1.0
P F:PEQ532 4.6 85.4 0.5
CA F:SER76 4.6 22.8 1.0
OD2 F:ASP112 4.6 5.0 1.0
CB F:ASP112 4.7 8.3 1.0
ND2 F:ASN74 4.8 47.0 1.0
CA F:ASP112 5.0 24.8 1.0

Potassium binding site 7 out of 8 in 1aqf

Go back to Potassium Binding Sites List in 1aqf
Potassium binding site 7 out of 8 in the Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 7 of Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate within 5.0Å range:
probe atom residue distance (Å) B Occ
G:K533

b:56.3
occ:1.00
 O2P G:PEQ532 2.8 37.7 0.5
OD1 G:ASN74 2.9 32.2 1.0
O G:THR113 2.9 46.4 1.0
O G:ASP112 3.0 44.4 1.0
OG G:SER76 3.1 0.0 1.0
OD1 G:ASP112 3.5 22.4 1.0
C G:THR113 3.6 35.6 1.0
CG G:ASP112 3.6 49.0 1.0
NZ G:LYS269 3.8 17.9 1.0
C G:ASP112 3.8 27.6 1.0
CG G:ASN74 3.8 17.4 1.0
OG G:SER242 3.9 31.1 1.0
OD2 G:ASP112 3.9 58.2 1.0
CA G:LYS114 4.0 82.2 1.0
N G:LYS114 4.1 46.9 1.0
CB G:ASP112 4.1 62.2 1.0
CB G:SER76 4.2 33.3 1.0
P G:PEQ532 4.2 75.1 0.5
ND2 G:ASN74 4.3 0.0 1.0
N G:SER76 4.4 43.1 1.0
N G:THR113 4.4 24.9 1.0
O2 G:PEQ532 4.5 43.9 0.5
NH2 G:ARG72 4.5 93.6 1.0
CA G:ASP112 4.6 42.4 1.0
CA G:THR113 4.6 7.5 1.0
O G:LYS114 4.7 74.3 1.0
CA G:SER76 4.7 58.9 1.0
CE G:LYS269 4.8 29.7 1.0
C G:LYS114 4.8 22.4 1.0
N G:PHE75 4.8 34.1 1.0
CB G:LYS114 4.9 45.5 1.0
O1P G:PEQ532 4.9 28.9 0.5

Potassium binding site 8 out of 8 in 1aqf

Go back to Potassium Binding Sites List in 1aqf
Potassium binding site 8 out of 8 in the Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 8 of Pyruvate Kinase From Rabbit Muscle with Mg, K, and L- Phospholactate within 5.0Å range:
probe atom residue distance (Å) B Occ
H:K533

b:83.5
occ:1.00
 O H:THR113 2.6 49.6 1.0
OD1 H:ASN74 3.0 51.0 1.0
O2P H:PEQ532 3.0 90.0 0.5
OG H:SER76 3.0 0.0 1.0
OD1 H:ASP112 3.3 49.8 1.0
O1P H:PEQ532 3.5 90.0 0.5
C H:THR113 3.6 25.5 1.0
CA H:LYS114 3.7 98.2 1.0
O H:LYS114 3.8 58.9 1.0
OG H:SER242 3.9 29.5 1.0
P H:PEQ532 3.9 88.7 0.5
NZ H:LYS269 4.0 10.6 1.0
CB H:SER76 4.0 36.3 1.0
CG H:ASN74 4.0 19.8 1.0
N H:LYS114 4.1 48.4 1.0
C H:LYS114 4.2 25.8 1.0
O H:ASP112 4.2 33.5 1.0
ND2 H:ASN74 4.4 0.0 1.0
CG H:ASP112 4.4 83.6 1.0
N H:SER76 4.5 78.5 1.0
CB H:LYS114 4.6 39.6 1.0
CA H:SER76 4.6 28.8 1.0
C H:ASP112 4.7 33.5 1.0
O2 H:PEQ532 4.9 47.1 0.5
CA H:THR113 4.9 41.5 1.0
O3P H:PEQ532 5.0 38.4 0.5

Reference:

T.M.Larsen, M.M.Benning, G.E.Wesenberg, I.Rayment, G.H.Reed. Ligand-Induced Domain Movement in Pyruvate Kinase: Structure of the Enzyme From Rabbit Muscle with MG2+, K+, and L-Phospholactate at 2.7 A Resolution. Arch.Biochem.Biophys. V. 345 199 1997.
ISSN: ISSN 0003-9861
PubMed: 9308890
DOI: 10.1006/ABBI.1997.0257
Page generated: Mon Aug 12 04:02:05 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy