Potassium in PDB 1a9x: Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis

The structure of Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis, PDB code: 1a9x was solved by J.Thoden, H.Holden, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	152.100, 164.400, 332.300, 90.00, 90.00, 90.00
R / Rfree (%)	19.1 / n/a

The structure of Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis also contains other interesting chemical elements:

Manganese	(Mn)	12 atoms
Chlorine	(Cl)	28 atoms

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 20; Page 3, Binding sites: 21 - 30; Page 4, Binding sites: 31 - 32;

Binding sites:

The binding sites of Potassium atom in the Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis (pdb code 1a9x). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 32 binding sites of Potassium where determined in the Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis, PDB code: 1a9x:
Jump to Potassium binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Potassium binding site 1 out of 32 in the Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis within 5.0Å range: probe atom residue distance (Å) B Occ A:K1903 b:18.8 occ:1.00 O A:ILE242 2.6 14.0 1.0 OE1 A:GLU215 2.7 16.8 1.0 OD1 A:ASN236 2.7 23.1 1.0 OG A:SER247 2.8 14.1 1.0 O A:ALA239 2.9 20.9 1.0 O A:ASP238 2.9 14.8 1.0 C A:ALA239 3.5 19.8 1.0 C A:ASP238 3.6 18.8 1.0 CG A:ASN236 3.6 34.4 1.0 CD A:GLU215 3.7 33.0 1.0 CB A:SER247 3.8 14.0 1.0 C A:ILE242 3.8 29.6 1.0 CB A:ASN236 3.9 15.9 1.0 CB A:GLU215 4.0 28.8 1.0 CG A:GLU215 4.2 17.9 1.0 N A:MET240 4.2 16.9 1.0 N A:ALA239 4.2 17.2 1.0 O2' A:ADP1900 4.3 21.6 1.0 CA A:ALA239 4.3 32.7 1.0 CB A:ASP238 4.3 13.5 1.0 CB A:ILE242 4.4 27.3 1.0 N A:ILE242 4.4 13.1 1.0 C A:HIS243 4.4 16.5 1.0 O A:HIS243 4.4 15.6 1.0 CA A:ILE242 4.4 12.7 1.0 N A:THR244 4.4 20.9 1.0 CA A:MET240 4.5 19.1 1.0 CA A:ASP238 4.5 18.1 1.0 CA A:THR244 4.6 11.2 1.0 OE2 A:GLU215 4.7 31.0 1.0 CG2 A:ILE242 4.8 6.5 1.0 CG2 A:THR244 4.8 18.0 1.0 N A:HIS243 4.8 13.9 1.0 ND2 A:ASN236 4.9 11.7 1.0 N A:ASP238 4.9 22.3 1.0

Potassium binding site 2 out of 32 in the Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis within 5.0Å range: probe atom residue distance (Å) B Occ A:K1904 b:28.1 occ:1.00 O A:MET300 2.6 18.0 1.0 OE1 A:GLU299 2.8 21.7 1.0 O A:HOH2674 2.9 25.8 1.0 OE2 A:GLU127 3.0 27.3 1.0 O A:HOH2081 3.2 18.7 1.0 O A:HOH2085 3.3 36.1 1.0 O A:ALA126 3.3 28.1 1.0 OD1 A:ASN301 3.6 16.8 1.0 CD A:GLU127 3.7 36.6 1.0 C A:MET300 3.7 19.4 1.0 MN A:MN1901 3.8 24.4 1.0 NH1 A:ARG129 3.9 34.5 1.0 CD A:GLU299 3.9 31.9 1.0 C A:ALA126 4.1 18.9 1.0 OE1 A:GLU127 4.1 23.4 1.0 N A:MET300 4.3 27.3 1.0 CZ A:ARG129 4.4 70.2 1.0 CA A:GLU127 4.4 17.8 1.0 CG A:ASN301 4.5 42.0 1.0 CB A:GLU299 4.5 14.0 1.0 N A:ASN301 4.5 16.7 1.0 O A:HOH2128 4.5 21.9 1.0 CA A:ASN301 4.6 14.5 1.0 N A:GLU127 4.6 23.5 1.0 CG A:GLU127 4.6 12.8 1.0 CA A:MET300 4.7 14.8 1.0 CG A:GLU299 4.8 19.2 1.0 NH2 A:ARG129 4.8 23.4 1.0 O A:HOH2084 4.8 20.7 1.0 OE2 A:GLU299 4.8 16.0 1.0 O3B A:ADP1900 4.8 18.4 1.0 CB A:ALA126 4.8 15.4 1.0 CD A:PRO302 4.9 14.9 1.0

Potassium binding site 3 out of 32 in the Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis within 5.0Å range: probe atom residue distance (Å) B Occ A:K1912 b:43.1 occ:1.00 OD1 A:ASN843 2.7 38.0 1.0 OE1 A:GLU841 2.8 47.8 1.0 O2B A:ADP1910 2.8 33.8 1.0 OE2 A:GLU841 2.8 37.9 1.0 CD A:GLU841 3.1 23.5 1.0 O3B A:ADP1910 3.3 26.2 1.0 O A:HOH2791 3.4 45.6 1.0 CG A:ASN843 3.5 39.4 1.0 O A:HOH2561 3.5 36.5 1.0 O A:HOH2789 3.7 38.9 1.0 ND2 A:ASN843 3.8 38.2 1.0 PB A:ADP1910 3.8 28.5 1.0 MN A:MN1911 3.9 34.4 1.0 NH2 A:ARG845 4.1 62.7 1.0 O A:HOH2351 4.2 13.1 1.0 O A:HOH2790 4.4 37.7 1.0 CG A:GLU841 4.5 32.6 1.0 O A:HOH2810 4.5 60.0 1.0 CB A:ASN843 4.8 14.2 1.0 O1B A:ADP1910 4.8 49.1 1.0 O2A A:ADP1910 4.9 30.6 1.0 O3A A:ADP1910 4.9 37.0 1.0

Potassium binding site 4 out of 32 in the Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis within 5.0Å range: probe atom residue distance (Å) B Occ A:K1913 b:21.4 occ:1.00 OE1 A:GLU761 2.6 19.5 1.0 O A:VAL787 2.7 13.5 1.0 OG A:SER792 2.8 20.9 1.0 ND1 A:HIS781 2.8 27.9 1.0 O A:GLN784 2.9 20.6 1.0 O A:GLU783 2.9 21.1 1.0 C A:GLN784 3.5 29.3 1.0 CD A:GLU761 3.6 38.2 1.0 CB A:GLU761 3.6 22.5 1.0 C A:GLU783 3.7 18.5 1.0 CE1 A:HIS781 3.7 19.7 1.0 CG A:HIS781 3.8 19.8 1.0 C A:VAL787 3.8 30.1 1.0 CB A:SER792 3.8 14.4 1.0 CB A:HIS781 3.9 18.3 1.0 N A:ALA785 4.0 16.0 1.0 CA A:ALA785 4.1 25.8 1.0 CB A:VAL787 4.1 23.0 1.0 CG A:GLU761 4.2 25.1 1.0 N A:VAL787 4.2 22.7 1.0 O2' A:ADP1910 4.3 26.7 1.0 N A:GLN784 4.3 22.4 1.0 CA A:VAL787 4.3 21.0 1.0 CA A:GLN784 4.3 23.4 1.0 O A:HIS788 4.4 19.7 1.0 CB A:GLU783 4.4 14.9 1.0 OE2 A:GLU761 4.5 43.4 1.0 C A:HIS788 4.5 24.3 1.0 CA A:GLU783 4.6 22.7 1.0 N A:SER789 4.6 23.9 1.0 CA A:SER789 4.6 16.9 1.0 CG1 A:VAL787 4.7 11.5 1.0 O A:HOH2358 4.7 12.6 1.0 C A:ALA785 4.8 29.8 1.0 N A:GLY786 4.8 20.1 1.0 NE2 A:HIS781 4.9 28.7 1.0 N A:HIS788 4.9 24.0 1.0 CD2 A:HIS781 4.9 27.0 1.0 CA A:GLU761 5.0 14.2 1.0

Potassium binding site 5 out of 32 in the Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 5 of Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis within 5.0Å range: probe atom residue distance (Å) B Occ A:K1940 b:38.4 occ:1.00 O A:GLY112 2.7 27.4 1.0 O A:ASP84 2.7 30.5 1.0 OG1 A:THR114 2.8 44.3 1.0 O A:HOH2628 2.9 59.4 1.0 O A:HOH2426 3.1 43.2 1.0 O A:HOH2615 3.5 54.9 1.0 N A:THR114 3.7 17.7 1.0 CB A:THR114 3.7 26.8 1.0 C A:ASP84 3.7 29.5 1.0 C A:GLY112 3.9 58.6 1.0 CA A:ASP84 4.0 16.9 1.0 CB A:ASP84 4.1 33.3 1.0 CA A:THR114 4.3 48.9 1.0 C A:VAL113 4.3 17.7 1.0 CA A:VAL113 4.4 41.6 1.0 OD1 A:ASP84 4.5 46.9 1.0 N A:VAL113 4.6 31.8 1.0 O A:HOH2046 4.7 41.7 1.0 CG A:ASP84 4.8 0.0 1.0 N A:ALA85 4.9 28.3 1.0 CA A:GLY112 5.0 27.3 1.0

Potassium binding site 6 out of 32 in the Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 6 of Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis within 5.0Å range: probe atom residue distance (Å) B Occ A:K1942 b:35.6 occ:1.00 OE1 A:GLU217 2.8 36.2 1.0 O A:HOH2124 2.9 22.2 1.0 OE1 A:GLN285 3.0 30.5 1.0 O2 A:PO41906 3.0 18.7 1.0 OD1 A:ASN283 3.2 58.1 1.0 OG1 A:THR244 3.2 24.4 1.0 O A:HOH2125 3.3 16.9 1.0 CD A:GLU217 3.5 0.0 1.0 CD A:GLN285 3.5 53.0 1.0 ND2 A:ASN283 3.6 65.6 1.0 OG A:SER307 3.6 25.4 1.0 CG A:ASN283 3.7 31.7 1.0 CG A:GLN285 3.7 22.0 1.0 MN A:MN1902 4.0 20.8 1.0 O4 A:PO41906 4.1 17.4 1.0 OE2 A:GLU217 4.2 32.5 1.0 P A:PO41906 4.2 29.5 1.0 ND2 A:ASN301 4.2 15.5 1.0 CG A:GLU217 4.2 42.7 1.0 CB A:THR244 4.2 19.2 1.0 CG2 A:THR244 4.4 18.0 1.0 NE2 A:GLN285 4.6 21.8 1.0 CB A:SER307 4.6 26.7 1.0 NH1 A:ARG306 4.8 22.2 1.0 OE2 A:GLU299 4.8 16.0 1.0 CB A:GLU217 4.9 16.6 1.0

Potassium binding site 7 out of 32 in the Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 7 of Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis within 5.0Å range: probe atom residue distance (Å) B Occ A:K1943 b:41.0 occ:1.00 O A:HOH2478 2.6 25.6 1.0 O A:THR143 3.0 19.2 1.0 O A:ALA144 3.1 22.7 1.0 OG1 A:THR143 3.1 38.0 1.0 O A:HOH2479 3.3 49.1 1.0 O A:HOH2477 3.4 30.1 1.0 C A:ALA144 3.6 30.2 1.0 C A:THR143 3.7 37.1 1.0 O A:HOH2112 3.9 36.4 1.0 CA A:ARG145 3.9 26.1 1.0 N A:ARG145 3.9 23.2 1.0 CB A:THR143 4.3 29.8 1.0 N A:ALA144 4.4 25.3 1.0 CA A:THR143 4.4 31.2 1.0 N A:THR143 4.5 25.2 1.0 CA A:ALA144 4.5 26.7 1.0 N A:SER146 4.6 33.1 1.0 O A:HOH2652 4.6 46.8 1.0 C A:ARG145 4.7 24.3 1.0 OD1 A:ASP133 4.8 61.8 1.0 OG A:SER146 4.8 38.7 1.0 CB A:ARG145 5.0 21.8 1.0

Potassium binding site 8 out of 32 in the Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 8 of Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis within 5.0Å range: probe atom residue distance (Å) B Occ B:K1941 b:39.0 occ:1.00 O B:HOH1984 2.6 37.4 1.0 O B:ASP1612 2.6 16.7 1.0 O B:HIS1516 2.7 29.3 1.0 O B:HOH2143 3.2 54.1 1.0 O A:HOH2288 3.4 33.5 1.0 O A:HOH2417 3.4 41.0 1.0 O B:HOH1977 3.6 52.6 1.0 C B:ASP1612 3.8 22.0 1.0 C B:HIS1516 3.8 32.0 1.0 CA B:GLY1517 4.1 14.9 1.0 CB B:ASP1612 4.3 19.3 1.0 NH2 A:ARG494 4.4 17.3 1.0 CA B:ILE1613 4.5 24.8 1.0 N B:GLY1517 4.5 25.4 1.0 CG2 B:ILE1613 4.5 24.4 1.0 O A:HOH2305 4.6 34.3 1.0 CL B:CL1933 4.6 31.7 1.0 N B:ILE1613 4.6 23.6 1.0 CA B:ASP1612 4.7 21.9 1.0 O B:HOH1972 4.7 30.2 1.0 CA B:HIS1516 4.9 17.3 1.0 CZ A:ARG494 4.9 18.9 1.0 O A:HOH2287 4.9 37.0 1.0

Potassium binding site 9 out of 32 in the Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 9 of Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis within 5.0Å range: probe atom residue distance (Å) B Occ C:K3903 b:14.8 occ:1.00 OE1 C:GLU2215 2.6 16.8 1.0 OD1 C:ASN2236 2.6 28.7 1.0 O C:ILE2242 2.6 15.5 1.0 OG C:SER2247 2.7 11.0 1.0 O C:ASP2238 2.9 19.1 1.0 O C:ALA2239 3.0 17.8 1.0 CG C:ASN2236 3.5 25.1 1.0 C C:ALA2239 3.6 27.8 1.0 C C:ASP2238 3.6 17.4 1.0 CD C:GLU2215 3.7 33.7 1.0 C C:ILE2242 3.7 24.5 1.0 CB C:SER2247 3.8 10.3 1.0 CB C:ASN2236 3.8 8.0 1.0 CB C:GLU2215 4.0 11.9 1.0 CG C:GLU2215 4.2 21.7 1.0 O2' C:ADP3900 4.3 14.8 1.0 N C:MET2240 4.3 18.5 1.0 N C:ALA2239 4.3 15.2 1.0 CB C:ASP2238 4.3 17.2 1.0 N C:ILE2242 4.3 15.5 1.0 C C:HIS2243 4.3 17.2 1.0 CB C:ILE2242 4.3 17.7 1.0 N C:THR2244 4.4 18.6 1.0 CA C:ALA2239 4.4 16.9 1.0 CA C:ILE2242 4.4 11.5 1.0 O C:HIS2243 4.5 18.4 1.0 CA C:ASP2238 4.5 11.6 1.0 CA C:MET2240 4.5 24.9 1.0 CA C:THR2244 4.5 10.1 1.0 OE2 C:GLU2215 4.6 28.4 1.0 N C:HIS2243 4.8 19.5 1.0 ND2 C:ASN2236 4.8 14.6 1.0 CG2 C:ILE2242 4.9 13.4 1.0 CG2 C:THR2244 5.0 13.2 1.0 CA C:HIS2243 5.0 8.6 1.0 C C:MET2240 5.0 18.1 1.0

Potassium binding site 10 out of 32 in the Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 10 of Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis within 5.0Å range: probe atom residue distance (Å) B Occ C:K3904 b:21.3 occ:1.00 OE1 C:GLU2299 2.6 15.8 1.0 O C:MET2300 2.7 16.0 1.0 O C:HOH4632 2.7 32.5 1.0 O C:HOH4079 3.0 22.6 1.0 OE2 C:GLU2127 3.0 27.4 1.0 O C:HOH4447 3.1 21.1 1.0 OD1 C:ASN2301 3.2 12.5 1.0 O C:ALA2126 3.3 25.4 1.0 O C:HOH4083 3.6 23.0 1.0 CD C:GLU2299 3.7 41.5 1.0 MN C:MN3901 3.7 19.6 1.0 C C:MET2300 3.7 21.9 1.0 CD C:GLU2127 3.7 0.0 1.0 C C:ALA2126 4.1 24.0 1.0 N C:MET2300 4.2 17.0 1.0 CG C:ASN2301 4.2 23.2 1.0 CB C:GLU2299 4.3 12.3 1.0 OE1 C:GLU2127 4.3 25.3 1.0 CA C:GLU2127 4.3 15.4 1.0 CA C:ASN2301 4.3 21.1 1.0 NH1 C:ARG2129 4.3 16.0 1.0 N C:ASN2301 4.4 15.8 1.0 O C:HOH4126 4.4 17.8 1.0 CG C:GLU2299 4.5 9.7 1.0 N C:GLU2127 4.5 15.8 1.0 OE2 C:GLU2299 4.6 15.1 1.0 CA C:MET2300 4.6 12.2 1.0 NH2 C:ARG2129 4.7 18.6 1.0 CZ C:ARG2129 4.7 36.4 1.0 O3B C:ADP3900 4.8 15.1 1.0 CD C:PRO2302 4.8 17.3 1.0 CG C:GLU2127 4.8 19.4 1.0 CB C:ALA2126 4.9 12.7 1.0 CB C:ASN2301 4.9 6.5 1.0

J.B.Thoden, S.G.Miran, J.C.Phillips, A.J.Howard, F.M.Raushel, H.M.Holden. Carbamoyl Phosphate Synthetase: Caught in the Act of Glutamine Hydrolysis. Biochemistry V. 37 8825 1998.
ISSN: ISSN 0006-2960
PubMed: 9636022
DOI: 10.1021/BI9807761