Potassium in PDB 1a5b: Cryo-Crystallography of A True Substrate, Indole-3-Glycerol Phosphate, Bound to A Mutant (Alpha D60N) Tryptophan Synthase ALPHA2BETA2 Complex Reveals the Correct Orientation of Active Site Alpha Glu 49

Enzymatic activity of Cryo-Crystallography of A True Substrate, Indole-3-Glycerol Phosphate, Bound to A Mutant (Alpha D60N) Tryptophan Synthase ALPHA2BETA2 Complex Reveals the Correct Orientation of Active Site Alpha Glu 49

All present enzymatic activity of Cryo-Crystallography of A True Substrate, Indole-3-Glycerol Phosphate, Bound to A Mutant (Alpha D60N) Tryptophan Synthase ALPHA2BETA2 Complex Reveals the Correct Orientation of Active Site Alpha Glu 49:
4.2.1.20;

Protein crystallography data

The structure of Cryo-Crystallography of A True Substrate, Indole-3-Glycerol Phosphate, Bound to A Mutant (Alpha D60N) Tryptophan Synthase ALPHA2BETA2 Complex Reveals the Correct Orientation of Active Site Alpha Glu 49, PDB code: 1a5b was solved by S.Rhee, E.W.Miles, D.R.Davies, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	8.00 / 2.00
*Space group*	C 1 2 1
*Cell size a, b, c (Å), α, β, γ (°)*	182.600, 59.700, 67.400, 90.00, 94.60, 90.00
*R / R_free (%)*	22 / 28.3

Potassium Binding Sites:

The binding sites of Potassium atom in the Cryo-Crystallography of A True Substrate, Indole-3-Glycerol Phosphate, Bound to A Mutant (Alpha D60N) Tryptophan Synthase ALPHA2BETA2 Complex Reveals the Correct Orientation of Active Site Alpha Glu 49 (pdb code 1a5b). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total only one binding site of Potassium was determined in the Cryo-Crystallography of A True Substrate, Indole-3-Glycerol Phosphate, Bound to A Mutant (Alpha D60N) Tryptophan Synthase ALPHA2BETA2 Complex Reveals the Correct Orientation of Active Site Alpha Glu 49, PDB code: 1a5b:

Potassium binding site 1 out of 1 in 1a5b

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Potassium Binding Sites List in 1a5b

Potassium binding site 1 out of 1 in the Cryo-Crystallography of A True Substrate, Indole-3-Glycerol Phosphate, Bound to A Mutant (Alpha D60N) Tryptophan Synthase ALPHA2BETA2 Complex Reveals the Correct Orientation of Active Site Alpha Glu 49

Mono view

Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Cryo-Crystallography of A True Substrate, Indole-3-Glycerol Phosphate, Bound to A Mutant (Alpha D60N) Tryptophan Synthase ALPHA2BETA2 Complex Reveals the Correct Orientation of Active Site Alpha Glu 49 within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
B:K500 b:41.4 occ:1.00	O	B:HOH596	2.6	34.1	1.0
	O	B:GLY232	2.6	22.2	1.0
	O	B:PHE306	2.6	31.2	1.0
	O	B:SER308	3.0	23.2	1.0
	O	B:GLY268	3.5	21.5	1.0
	C	B:PHE306	3.7	30.1	1.0
	C	B:GLY232	3.7	22.1	1.0
	CD	B:PRO270	3.8	20.9	1.0
	CB	B:PHE306	3.9	30.1	1.0
	O	B:LEU304	4.0	31.9	1.0
	CG	B:PRO270	4.0	18.8	1.0
	O	B:VAL231	4.1	17.5	1.0
	C	B:SER308	4.2	19.5	1.0
	CA	B:PHE306	4.2	29.7	1.0
	N	B:PHE306	4.2	31.2	1.0
	CA	B:GLY232	4.2	19.9	1.0
	CG1	B:VAL309	4.3	26.0	1.0
	C	B:GLY268	4.5	19.7	1.0
	N	B:SER308	4.5	20.5	1.0
	CD2	B:PHE306	4.7	31.3	1.0
	OE2	B:GLU256	4.7	19.9	1.0
	CG	B:PHE306	4.8	31.6	1.0
	OG	B:SER297	4.8	41.8	1.0
	N	B:PRO307	4.8	30.5	1.0
	C	B:VAL231	4.8	16.3	1.0
	N	B:GLY233	4.9	18.2	1.0
	CA	B:SER308	4.9	20.6	1.0
	C	B:PRO307	4.9	24.6	1.0
	C	B:LEU304	4.9	27.9	1.0
	N	B:GLY232	5.0	18.0	1.0

Reference:

S.Rhee, E.W.Miles, D.R.Davies. Cryo-Crystallography of A True Substrate, Indole-3-Glycerol Phosphate, Bound to A Mutant (ALPHAD60N) Tryptophan Synthase ALPHA2BETA2 Complex Reveals the Correct Orientation of Active Site ALPHAGLU49. J.Biol.Chem. V. 273 8553 1998.
ISSN: ISSN 0021-9258
PubMed: 9535826
DOI: 10.1074/JBC.273.15.8553

Page generated: Mon Aug 12 04:00:29 2024

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