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Potassium in PDB 8h5j: Crystal Structure of Petase S121E/A180V/P181V/D186H/N233C/S242T/N246D/S282C Mutant From Ideonella Sakaiensis

Enzymatic activity of Crystal Structure of Petase S121E/A180V/P181V/D186H/N233C/S242T/N246D/S282C Mutant From Ideonella Sakaiensis

All present enzymatic activity of Crystal Structure of Petase S121E/A180V/P181V/D186H/N233C/S242T/N246D/S282C Mutant From Ideonella Sakaiensis:
3.1.1.101;

Protein crystallography data

The structure of Crystal Structure of Petase S121E/A180V/P181V/D186H/N233C/S242T/N246D/S282C Mutant From Ideonella Sakaiensis, PDB code: 8h5j was solved by S.H.Lee, H.Seo, K.-J.Kim, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 28.65 / 1.40
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 139.218, 50.665, 80.854, 90, 124.64, 90
R / Rfree (%) 15.7 / 19

Potassium Binding Sites:

The binding sites of Potassium atom in the Crystal Structure of Petase S121E/A180V/P181V/D186H/N233C/S242T/N246D/S282C Mutant From Ideonella Sakaiensis (pdb code 8h5j). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 3 binding sites of Potassium where determined in the Crystal Structure of Petase S121E/A180V/P181V/D186H/N233C/S242T/N246D/S282C Mutant From Ideonella Sakaiensis, PDB code: 8h5j:
Jump to Potassium binding site number: 1; 2; 3;

Potassium binding site 1 out of 3 in 8h5j

Go back to Potassium Binding Sites List in 8h5j
Potassium binding site 1 out of 3 in the Crystal Structure of Petase S121E/A180V/P181V/D186H/N233C/S242T/N246D/S282C Mutant From Ideonella Sakaiensis


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Petase S121E/A180V/P181V/D186H/N233C/S242T/N246D/S282C Mutant From Ideonella Sakaiensis within 5.0Å range:
probe atom residue distance (Å) B Occ
A:K401

b:18.1
occ:1.00
 NH1 A:ARG132 3.4 15.6 0.5
CD A:ARG132 3.5 20.2 0.5
CD A:ARG132 3.6 14.7 0.5
NH2 A:ARG132 3.9 23.0 0.5
CZ A:ARG132 4.2 15.6 0.5
NE A:ARG132 4.2 21.0 0.5
NE A:ARG132 4.3 14.0 0.5
CZ A:ARG132 4.4 21.0 0.5
CG A:ARG132 4.6 13.3 0.5
CG A:ARG132 4.6 17.7 0.5

Potassium binding site 2 out of 3 in 8h5j

Go back to Potassium Binding Sites List in 8h5j
Potassium binding site 2 out of 3 in the Crystal Structure of Petase S121E/A180V/P181V/D186H/N233C/S242T/N246D/S282C Mutant From Ideonella Sakaiensis


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Petase S121E/A180V/P181V/D186H/N233C/S242T/N246D/S282C Mutant From Ideonella Sakaiensis within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K401

b:26.5
occ:1.00
 O B:HOH604 2.8 30.6 1.0
OG B:SER160 2.9 20.3 1.0
N B:TYR87 3.2 16.3 1.0
N B:MET161 3.3 14.8 1.0
CB B:SER160 3.3 15.8 1.0
CG B:MET161 3.4 18.4 1.0
CB B:TYR87 3.5 17.6 1.0
CB B:MET161 3.7 15.5 1.0
CA B:GLY86 3.9 16.3 1.0
CA B:TYR87 4.0 16.3 1.0
CA B:MET161 4.1 14.5 1.0
C B:GLY86 4.1 15.2 1.0
SD B:MET161 4.2 19.4 1.0
O B:HOH620 4.2 27.8 1.0
C B:SER160 4.2 14.6 1.0
CA B:SER160 4.3 14.4 1.0
CE B:MET161 4.4 23.8 1.0
O B:TYR87 4.8 16.9 1.0
NE2 B:HIS237 4.8 18.3 1.0
CG B:TYR87 4.8 17.4 1.0
C B:TYR87 4.9 16.1 1.0

Potassium binding site 3 out of 3 in 8h5j

Go back to Potassium Binding Sites List in 8h5j
Potassium binding site 3 out of 3 in the Crystal Structure of Petase S121E/A180V/P181V/D186H/N233C/S242T/N246D/S282C Mutant From Ideonella Sakaiensis


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Crystal Structure of Petase S121E/A180V/P181V/D186H/N233C/S242T/N246D/S282C Mutant From Ideonella Sakaiensis within 5.0Å range:
probe atom residue distance (Å) B Occ
B:K404

b:43.4
occ:1.00
 NH1 B:ARG267 2.8 27.2 1.0
O B:HOH659 2.8 27.9 1.0
CD B:ARG267 3.4 23.2 1.0
CB B:ASN37 3.4 24.6 1.0
N B:ASN37 3.5 25.2 1.0
CA B:ASN37 3.6 26.6 1.0
CZ B:ARG267 3.8 27.9 1.0
NE B:ARG267 4.1 24.1 1.0
O B:HOH753 4.1 44.2 1.0
CG B:ASN37 4.1 28.8 1.0
C B:PRO36 4.4 28.6 1.0
OD1 B:ASN37 4.5 23.5 1.0
CG B:ARG267 4.7 24.0 1.0
ND2 B:ASN37 4.7 25.7 1.0
O B:GLY35 4.9 26.6 1.0

Reference:

S.H.Lee, H.Seo, K.J.Kim. A Case of Balance Engineering Exhibits Kinetic Relationship Between Mesophilic and Thermophilic Poly(Ethylene Terephthalate) Depolymerases. To Be Published.
Page generated: Mon Aug 12 23:52:26 2024

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