Potassium in PDB 7s1y: Cryo-Em Structure of Human NKCC1 K289NA492E Bound with Bumetanide

The structure of Cryo-Em Structure of Human NKCC1 K289NA492E Bound with Bumetanide also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Potassium atom in the Cryo-Em Structure of Human NKCC1 K289NA492E Bound with Bumetanide (pdb code 7s1y). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Cryo-Em Structure of Human NKCC1 K289NA492E Bound with Bumetanide, PDB code: 7s1y:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Cryo-Em Structure of Human NKCC1 K289NA492E Bound with Bumetanide


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Cryo-Em Structure of Human NKCC1 K289NA492E Bound with Bumetanide within 5.0Å range: probe atom residue distance (Å) B Occ A:K1301 b:30.0 occ:1.00 O5 A:82U1303 1.6 126.2 1.0 C17 A:82U1303 2.4 126.2 1.0 O A:ALA497 2.6 131.2 1.0 O A:PRO496 2.6 126.7 1.0 OH A:TYR383 2.7 120.6 1.0 CA A:ALA497 2.9 131.2 1.0 O4 A:82U1303 2.9 126.2 1.0 C A:ALA497 3.0 131.2 1.0 O A:THR499 3.2 124.8 1.0 CG A:82U1303 3.4 126.2 1.0 C A:PRO496 3.5 126.7 1.0 N A:ALA497 3.6 131.2 1.0 CB A:82U1303 3.6 126.2 1.0 CZ A:TYR383 3.7 120.6 1.0 O A:ILE299 3.8 125.9 1.0 CE1 A:TYR383 4.0 120.6 1.0 CA A:GLY301 4.0 125.3 1.0 CB A:ALA497 4.0 131.2 1.0 O A:ASN298 4.0 125.3 1.0 N A:GLY301 4.2 125.3 1.0 N A:ALA498 4.2 124.2 1.0 C A:THR499 4.4 124.8 1.0 N A:THR499 4.5 124.8 1.0 C A:ILE299 4.5 125.9 1.0 CD2 A:82U1303 4.7 126.2 1.0 CG2 A:THR499 4.8 124.8 1.0 CA A:PRO496 4.9 126.7 1.0 CE2 A:TYR383 4.9 120.6 1.0 CA A:ILE299 4.9 125.9 1.0 CA A:82U1303 5.0 126.2 1.0

Potassium binding site 2 out of 2 in the Cryo-Em Structure of Human NKCC1 K289NA492E Bound with Bumetanide


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Cryo-Em Structure of Human NKCC1 K289NA492E Bound with Bumetanide within 5.0Å range: probe atom residue distance (Å) B Occ B:K1301 b:30.0 occ:1.00 O5 B:82U1303 1.6 132.3 1.0 C17 B:82U1303 2.4 132.3 1.0 O B:PRO496 2.6 132.2 1.0 O B:ALA497 2.7 136.2 1.0 OH B:TYR383 2.8 122.7 1.0 CA B:ALA497 3.1 136.2 1.0 C B:ALA497 3.1 136.2 1.0 O4 B:82U1303 3.1 132.3 1.0 O B:THR499 3.2 127.3 1.0 CG B:82U1303 3.4 132.3 1.0 CB B:82U1303 3.5 132.3 1.0 C B:PRO496 3.6 132.2 1.0 N B:ALA497 3.8 136.2 1.0 CZ B:TYR383 3.8 122.7 1.0 O B:ILE299 3.9 126.2 1.0 O B:ASN298 4.0 123.1 1.0 CA B:GLY301 4.1 126.8 1.0 CE1 B:TYR383 4.1 122.7 1.0 N B:GLY301 4.3 126.8 1.0 CB B:ALA497 4.3 136.2 1.0 N B:ALA498 4.3 127.0 1.0 C B:THR499 4.4 127.3 1.0 N B:THR499 4.4 127.3 1.0 C B:ILE299 4.6 126.2 1.0 CG2 B:THR499 4.7 127.3 1.0 CD2 B:82U1303 4.8 132.3 1.0 CA B:82U1303 4.8 132.3 1.0 CA B:ILE299 4.9 126.2 1.0 C6 B:82U1303 4.9 132.3 1.0 CE2 B:TYR383 5.0 122.7 1.0 CA B:PRO496 5.0 132.2 1.0 CA B:THR499 5.0 127.3 1.0

Y.Zhao, K.Roy, P.Vidossich, L.Cancedda, M.De Vivo, B.Forbush, E.Cao. Structural Basis For Inhibition of the Cation-Chloride Cotransporter NKCC1 By the Diuretic Drug Bumetanide Nat Commun V. 13 2747 2022.
ISSN: ESSN 2041-1723
DOI: 10.1038/S41467-022-30407-3