Potassium in PDB 7r97: Crystal Structure of Postcleavge Complex of Escherichia Coli Rnase III

All present enzymatic activity of Crystal Structure of Postcleavge Complex of Escherichia Coli Rnase III:
3.1.26.3;

The structure of Crystal Structure of Postcleavge Complex of Escherichia Coli Rnase III, PDB code: 7r97 was solved by S.Dharavath, G.X.Shaw, X.Ji, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.31 / 1.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	56.929, 65.753, 84.519, 90, 102.09, 90
R / Rfree (%)	17.1 / 20.2

The structure of Crystal Structure of Postcleavge Complex of Escherichia Coli Rnase III also contains other interesting chemical elements:

Chlorine	(Cl)	7 atoms
Magnesium	(Mg)	6 atoms

The binding sites of Potassium atom in the Crystal Structure of Postcleavge Complex of Escherichia Coli Rnase III (pdb code 7r97). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 2 binding sites of Potassium where determined in the Crystal Structure of Postcleavge Complex of Escherichia Coli Rnase III, PDB code: 7r97:
Jump to Potassium binding site number: 1; 2;

Potassium binding site 1 out of 2 in the Crystal Structure of Postcleavge Complex of Escherichia Coli Rnase III


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Crystal Structure of Postcleavge Complex of Escherichia Coli Rnase III within 5.0Å range: probe atom residue distance (Å) B Occ C:K102 b:26.3 occ:1.00 O6 C:G21 2.7 19.9 1.0 O C:HOH266 2.8 26.8 1.0 O4 C:U6 2.8 17.6 1.0 O6 C:G20 2.9 18.6 1.0 O C:HOH210 2.9 23.0 1.0 O C:HOH289 2.9 44.0 1.0 O C:HOH204 3.0 26.8 1.0 O6 C:G5 3.3 17.4 1.0 O C:HOH244 3.4 24.5 1.0 C6 C:G20 3.7 17.3 1.0 C4 C:U6 3.7 19.8 1.0 C6 C:G21 3.8 19.2 1.0 N3 C:U6 3.9 19.4 1.0 N4 C:C22 3.9 17.0 1.0 N7 C:G21 4.2 18.5 1.0 N4 C:C7 4.2 18.8 1.0 O C:HOH227 4.3 27.4 1.0 C5 C:G21 4.4 18.5 1.0 C6 C:G5 4.4 19.1 1.0 C5 C:G20 4.4 16.3 1.0 N7 C:G20 4.5 18.4 1.0 O C:HOH214 4.5 30.2 1.0 H22 C:EDO105 4.5 43.9 1.0 N1 C:G20 4.6 18.4 1.0 C4 C:C22 4.8 18.7 1.0 N1 C:G21 5.0 18.6 1.0

Potassium binding site 2 out of 2 in the Crystal Structure of Postcleavge Complex of Escherichia Coli Rnase III


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Crystal Structure of Postcleavge Complex of Escherichia Coli Rnase III within 5.0Å range: probe atom residue distance (Å) B Occ D:K102 b:24.9 occ:1.00 HO2 D:EDO105 2.2 36.3 1.0 O6 D:G21 2.7 19.9 1.0 O D:HOH245 2.8 25.3 1.0 O6 D:G20 2.8 18.8 1.0 O4 D:U6 2.9 18.7 1.0 O D:HOH225 2.9 21.2 1.0 O2 D:EDO105 3.0 30.3 1.0 H21 D:EDO104 3.1 42.3 1.0 O6 D:G5 3.3 18.1 1.0 O D:HOH231 3.5 25.0 1.0 H11 D:EDO104 3.6 33.4 1.0 C6 D:G20 3.6 16.5 1.0 C6 D:G21 3.8 18.9 1.0 C4 D:U6 3.8 20.7 1.0 N4 D:C22 3.8 16.8 1.0 N3 D:U6 3.9 14.9 1.0 C2 D:EDO104 4.0 35.2 1.0 H22 D:EDO105 4.0 43.7 1.0 C2 D:EDO105 4.1 36.4 1.0 HO2 D:EDO104 4.2 30.2 1.0 N7 D:G21 4.2 20.6 1.0 C1 D:EDO104 4.3 27.8 1.0 N4 D:C7 4.3 15.9 1.0 C5 D:G20 4.3 16.1 1.0 C5 D:G21 4.3 15.7 1.0 C6 D:G5 4.4 17.8 1.0 N7 D:G20 4.4 18.9 1.0 O2 D:EDO104 4.5 25.2 1.0 N1 D:G20 4.5 18.2 1.0 H12 D:EDO104 4.6 33.4 1.0 H12 D:EDO105 4.7 60.2 1.0 C4 D:C22 4.8 18.4 1.0 H21 D:EDO105 4.8 43.7 1.0 H22 D:EDO104 4.8 42.3 1.0 N1 D:G21 4.9 20.1 1.0

S.Dharavath, G.X.Shaw, X.Ji. Structural Basis For Dicer-Like Function of An Engineered Rnase III Variant and Insights Into the Reaction Trajectory of Two-Mg 2+ -Ion Catalysis. Rna Biol. V. 19 908 2022.
ISSN: ESSN 1555-8584
PubMed: 35829618
DOI: 10.1080/15476286.2022.2099650