Potassium in PDB 7oou: Nak C-Di Mutant with Li+ and K+

The structure of Nak C-Di Mutant with Li+ and K+, PDB code: 7oou was solved by S.Minniberger, A.J.R.Plested, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.21 / 1.80
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	81.481, 88.413, 49.586, 90, 90, 90
R / Rfree (%)	17.6 / 20.6

The structure of Nak C-Di Mutant with Li+ and K+ also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Potassium atom in the Nak C-Di Mutant with Li+ and K+ (pdb code 7oou). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 4 binding sites of Potassium where determined in the Nak C-Di Mutant with Li+ and K+, PDB code: 7oou:
Jump to Potassium binding site number: 1; 2; 3; 4;

Potassium binding site 1 out of 4 in the Nak C-Di Mutant with Li+ and K+


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of Nak C-Di Mutant with Li+ and K+ within 5.0Å range: probe atom residue distance (Å) B Occ A:K201 b:22.1 occ:0.50 OG1 A:THR63 2.8 16.4 1.0 OG1 B:THR63 2.8 18.8 1.0 O A:THR63 2.8 18.6 1.0 O B:THR63 2.8 17.9 1.0 HB A:THR63 3.0 29.5 1.0 HB B:THR63 3.0 23.0 1.0 CB A:THR63 3.4 24.6 1.0 CB B:THR63 3.4 19.2 1.0 HG1 A:THR63 3.5 19.7 1.0 HG1 B:THR63 3.5 22.6 1.0 K A:K202 3.5 20.7 0.5 C A:THR63 3.7 16.8 1.0 C B:THR63 3.7 18.1 1.0 CA A:THR63 4.2 17.1 1.0 CA B:THR63 4.2 17.7 1.0 HA B:VAL64 4.4 20.4 1.0 HA A:VAL64 4.5 21.9 1.0 HG21 B:THR63 4.6 22.9 1.0 HG21 A:THR63 4.6 23.6 1.0 CG2 A:THR63 4.7 19.7 1.0 N B:VAL64 4.7 16.7 1.0 CG2 B:THR63 4.7 19.1 1.0 N A:VAL64 4.7 18.1 1.0 HA A:THR63 4.7 20.6 1.0 HA B:THR63 4.7 21.3 1.0 O A:THR62 5.0 18.7 1.0

Potassium binding site 2 out of 4 in the Nak C-Di Mutant with Li+ and K+


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of Nak C-Di Mutant with Li+ and K+ within 5.0Å range: probe atom residue distance (Å) B Occ A:K202 b:20.7 occ:0.50 O A:THR63 2.8 18.6 1.0 O B:THR63 2.8 17.9 1.0 O B:VAL64 2.9 20.8 1.0 O A:VAL64 3.0 19.7 1.0 K A:K204 3.2 47.0 0.3 K A:K201 3.5 22.1 0.5 C B:VAL64 3.6 18.6 1.0 C A:VAL64 3.6 20.7 1.0 HA A:VAL64 3.8 21.9 1.0 HA B:VAL64 3.8 20.4 1.0 C A:THR63 3.9 16.8 1.0 HA3 B:GLY65 4.0 26.9 1.0 C B:THR63 4.0 18.1 1.0 HA3 A:GLY65 4.1 27.6 1.0 CA A:VAL64 4.2 18.3 1.0 CA B:VAL64 4.2 17.0 1.0 N A:GLY65 4.4 19.3 1.0 N B:GLY65 4.4 18.7 1.0 N A:VAL64 4.6 18.1 1.0 N B:VAL64 4.6 16.7 1.0 CA B:GLY65 4.7 22.4 1.0 HB A:THR63 4.7 29.5 1.0 HB B:THR63 4.8 23.0 1.0 CA A:GLY65 4.8 23.0 1.0 H A:GLY65 5.0 23.2 1.0

Potassium binding site 3 out of 4 in the Nak C-Di Mutant with Li+ and K+


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of Nak C-Di Mutant with Li+ and K+ within 5.0Å range: probe atom residue distance (Å) B Occ A:K203 b:94.3 occ:0.50 K A:K204 3.0 47.0 0.3 O A:CYS66 3.5 39.0 1.0 O B:CYS66 3.7 47.8 1.0 O B:HOH319 4.0 40.9 1.0 C A:CYS66 4.2 37.3 1.0 HA A:ASP67 4.2 44.5 1.0 HA B:ASP67 4.3 44.5 1.0 H A:CYS66 4.4 37.2 1.0 H B:CYS66 4.5 49.6 1.0 C B:CYS66 4.5 35.9 1.0 HA A:CYS66 4.6 42.5 1.0 N A:CYS66 4.7 31.1 1.0 CA A:CYS66 4.8 35.4 1.0 N B:CYS66 4.9 41.4 1.0 N A:ASP67 5.0 33.5 1.0 HA3 A:GLY65 5.0 27.6 1.0

Potassium binding site 4 out of 4 in the Nak C-Di Mutant with Li+ and K+


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 4 of Nak C-Di Mutant with Li+ and K+ within 5.0Å range: probe atom residue distance (Å) B Occ A:K204 b:47.0 occ:0.26 K A:K203 3.0 94.3 0.5 K A:K202 3.2 20.7 0.5 HA3 B:GLY65 3.3 26.9 1.0 O A:VAL64 3.3 19.7 1.0 HA3 A:GLY65 3.3 27.6 1.0 O B:VAL64 3.4 20.8 1.0 O B:HOH319 3.7 40.9 1.0 CA B:GLY65 4.2 22.4 1.0 CA A:GLY65 4.2 23.0 1.0 C A:VAL64 4.4 20.7 1.0 C B:VAL64 4.4 18.6 1.0 H B:CYS66 4.5 49.6 1.0 H A:CYS66 4.5 37.2 1.0 C A:GLY65 4.6 26.0 1.0 C B:GLY65 4.6 28.4 1.0 N A:CYS66 4.7 31.1 1.0 N A:GLY65 4.8 19.3 1.0 N B:CYS66 4.8 41.4 1.0 N B:GLY65 4.8 18.7 1.0 HA2 B:GLY65 4.8 26.9 1.0 HA2 A:GLY65 4.9 27.6 1.0

S.Minniberger, S.Abdolvand, S.Braunbeck, H.Sun, A.J.R.Plested. Asymmetry and Ion Selectivity Properties in the Bacterial Channel Nak Mimicking Ionotropic Glutamate Receptors To Be Published.