Potassium in PDB 7lo2: S-Adenosylmethionine Synthetase Cocrystallized with Ctp

All present enzymatic activity of S-Adenosylmethionine Synthetase Cocrystallized with Ctp:
2.5.1.6;

The structure of S-Adenosylmethionine Synthetase Cocrystallized with Ctp, PDB code: 7lo2 was solved by L.L.Tan, C.J.Jackson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	38.22 / 1.89
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	100.139, 118.32, 144.456, 90, 90, 90
R / Rfree (%)	16.6 / 19.5

The structure of S-Adenosylmethionine Synthetase Cocrystallized with Ctp also contains other interesting chemical elements:

Magnesium

(Mg)

8 atoms

The binding sites of Potassium atom in the S-Adenosylmethionine Synthetase Cocrystallized with Ctp (pdb code 7lo2). This binding sites where shown within 5.0 Angstroms radius around Potassium atom.
In total 3 binding sites of Potassium where determined in the S-Adenosylmethionine Synthetase Cocrystallized with Ctp, PDB code: 7lo2:
Jump to Potassium binding site number: 1; 2; 3;

Potassium binding site 1 out of 3 in the S-Adenosylmethionine Synthetase Cocrystallized with Ctp


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 1 of S-Adenosylmethionine Synthetase Cocrystallized with Ctp within 5.0Å range: probe atom residue distance (Å) B Occ B:K403 b:70.4 occ:1.00 OE2 A:GLU42 2.5 47.0 1.0 O4 B:POP405 2.9 30.9 1.0 O B:CYS239 3.3 36.0 0.5 O B:HOH518 3.3 30.7 1.0 O B:CYS239 3.4 35.8 0.5 CD A:GLU42 3.6 44.0 1.0 O A:HOH641 3.6 33.4 1.0 O B:HOH557 3.6 29.1 1.0 O B:HOH629 3.7 45.8 1.0 O5 B:POP405 3.8 35.9 1.0 OD1 B:ASP238 3.9 38.0 0.5 P2 B:POP405 3.9 36.4 1.0 MG B:MG401 3.9 27.8 1.0 CG A:GLU42 3.9 34.9 1.0 O B:HOH508 3.9 50.5 1.0 CB B:CYS239 4.1 36.8 0.5 N B:CYS239 4.2 38.1 0.5 C B:CYS239 4.2 38.5 0.5 N B:CYS239 4.2 37.5 0.5 C B:CYS239 4.3 38.5 0.5 CB B:CYS239 4.3 36.9 0.5 NH2 B:ARG244 4.3 31.6 1.0 O B:HOH515 4.4 34.6 1.0 CA B:CYS239 4.4 37.6 0.5 CA B:CYS239 4.5 37.7 0.5 O B:HOH512 4.7 43.1 1.0 OE1 A:GLU42 4.7 39.3 1.0 O B:POP405 4.9 35.9 1.0 C B:ASP238 5.0 42.5 0.5

Potassium binding site 2 out of 3 in the S-Adenosylmethionine Synthetase Cocrystallized with Ctp


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 2 of S-Adenosylmethionine Synthetase Cocrystallized with Ctp within 5.0Å range: probe atom residue distance (Å) B Occ B:K404 b:79.9 occ:1.00 OE2 B:GLU42 2.6 52.1 1.0 O A:HOH505 3.1 41.4 1.0 O B:HOH595 3.3 49.0 1.0 O B:HOH627 3.4 51.4 1.0 O A:CYS239 3.4 38.0 0.5 CD B:GLU42 3.5 43.8 1.0 O A:HOH531 3.7 40.5 1.0 O A:CYS239 3.7 37.8 0.5 CG B:GLU42 3.7 38.0 1.0 OD1 A:ASP238 3.7 49.6 1.0 O A:HOH532 3.8 35.6 1.0 CB A:CYS239 3.9 37.7 0.5 CB A:CYS239 4.2 37.5 0.5 MG A:MG401 4.3 39.9 1.0 O A:HOH656 4.3 50.6 1.0 N A:CYS239 4.3 36.2 0.5 N A:CYS239 4.3 36.2 0.5 C A:CYS239 4.4 35.7 0.5 C A:CYS239 4.4 35.8 0.5 CA A:CYS239 4.4 36.4 0.5 O B:HOH637 4.5 45.0 1.0 CA A:CYS239 4.5 36.3 0.5 OE1 B:GLU42 4.6 43.0 1.0 CB B:ALA40 4.7 38.2 1.0 NH2 A:ARG244 4.9 33.5 1.0 CG A:ASP238 5.0 49.7 1.0

Potassium binding site 3 out of 3 in the S-Adenosylmethionine Synthetase Cocrystallized with Ctp


Mono view


Stereo pair view

A full contact list of Potassium with other atoms in the K binding site number 3 of S-Adenosylmethionine Synthetase Cocrystallized with Ctp within 5.0Å range: probe atom residue distance (Å) B Occ C:K404 b:81.2 occ:1.00 OE2 D:GLU42 2.7 45.9 1.0 O2 C:POP405 2.8 57.2 0.6 O D:HOH603 2.9 45.9 1.0 O3 C:POP405 3.3 43.5 0.6 O C:CYS239 3.3 43.0 0.6 O C:HOH579 3.3 49.1 1.0 P1 C:POP405 3.4 66.6 0.6 CD D:GLU42 3.5 42.7 1.0 O1 C:POP405 3.5 64.7 0.6 CG D:GLU42 3.6 40.0 1.0 CB C:CYS239 3.6 40.2 0.4 O C:HOH645 3.8 55.8 1.0 O C:CYS239 3.8 41.9 0.4 O C:HOH524 3.9 37.7 1.0 OD1 C:ASP238 3.9 53.2 1.0 O C:HOH518 4.0 32.9 1.0 CB C:CYS239 4.0 38.2 0.6 N C:CYS239 4.2 40.9 0.4 C C:CYS239 4.2 39.4 0.6 N C:CYS239 4.3 40.9 0.6 CA C:CYS239 4.3 38.1 0.4 C C:CYS239 4.3 39.7 0.4 CA C:CYS239 4.4 37.9 0.6 O C:HOH501 4.5 46.1 1.0 MG C:MG401 4.6 43.1 1.0 CB D:ALA40 4.7 38.0 1.0 SG C:CYS239 4.7 45.7 0.4 OE1 D:GLU42 4.7 37.0 1.0 O D:ALA40 4.9 40.1 1.0 SG C:CYS239 4.9 39.7 0.6

P.Laurino, C.J.Jackson. S-Adenosylmethionine Synthetase To Be Published.